Calmegin/CLGN Protein, Human (HEK293, His)

Calmegin Protein, Human (HEK293, His), a recombinant human Calmegin produced in HEK293 cells, has a His tag at the N-terminus. Calmegin is a testis-specific molecular chaperon playing a key role in spermatogenesis^[1].

Calmegin is a testis-specific molecular chaperon playing a key role in spermatogenesis. calmegin expression is regulated by HDAC and CpG methyltransferase in a coordinative way^[1].

Measured by its binding ability in a functional ELISA. Immobilized Huamn CLGN at 2 μg/mL (100 μL/Well) can bind Anti-CLGN Antibody. The ED₅₀ for this effect is 0.1 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Huamn CLGN at 2 μg/mL (100 μL/Well) can bind Anti-CLGN Antibody. The ED₅₀ for this effect is 0.1 μg/mL.

Human

HEK293

C-6*His

O14967-1 (E20-W471)

1047  [NCBI]

Lumenal Domain

EFMDDDVETEDFEENSEEIDVNESELSSEIKYKTPQPIGEVYFAETFDSGRLAGWVLSKAKKDDMDEEISIYDGRWEIEELKENQVPGDRGLVLKSRAKHHAISAVLAKPFIFADKPLIVQYEVNFQDGIDCGGAYIKLLADTDDLILENFYDKTSYIIMFGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPW

Approximately 60-63 kDa

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  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Dong Hoon Kim, et al. Coordinated transcriptional regulation of calmegin,a testis-specific molecular chaperon, by histone deacetylase and CpG methyltransferase. Exp Mol Med. 2005 Oct 31;37(5):492-6.  [Content Brief]