Calmodulin Protein, Human
Based on 1 publication(s) in Google Scholar
Calmodulin Protein, Human is a recombinant human Calmodulin expressed in E. coli. Calmodulin is a low molecular weight, acidic, calcium binding protein which mediates the Ca2+ regulation of a wide range of physiological processes throughout eukaryotic organisms.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Calmodulin Protein, Human is a recombinant human Calmodulin expressed in E. coli. Calmodulin is a low molecular weight, acidic, calcium binding protein which mediates the Ca2+ regulation of a wide range of physiological processes throughout eukaryotic organisms[1].
At low free Ca2+ concentrations, such as exist in resting muscle sarcoplasm, calmodulin exists in the Ca2+-free form in which state it does not generally interact with a target protein. Following an appropriate stimulus, the free Ca2+ concentration rises whereupon Ca2+ binds to calmodulin which undergoes a conformational change enabling it to interact with a target protein(s)[1].
206875- 251818.182 units per mg protein. One unit is defined as the amount of calmodulin which will give rise to 50% of the maximal enzyme activation of a standard level of activator-deficient calcineurin.
Publications (1)
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Journal Impact Factor
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Most Recent
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J Neurosci
CaMKIIβ-mediated Phosphorylation Enhances Protein Stability of Spastin to Promote Neurite Outgrowth. [Abstract]2025 Aug 6;45(32):e1995242025. PMID: 40645772
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
P0DP23 (M1-K149)
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Molecular Construction
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N-term
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Calmodulin (M1-K149)
Accession # P0DP23 -
C-term
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Protein Length
Full Length
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Synonyms
rHuCalmodulin; Calmodulin; CaM; CALM1
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AA Sequence
MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
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Molecular Weight
Approximately 19 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
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≥ 95%, as determined by reducing SDS-PAGE.
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Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 50 mM NH4HCO3, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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