CAPZA2 Protein, Human (His-SUMO)
Based on 1 Customer Validation
The CAPZA2 protein is a member of the F-actin capping protein family and binds to the barbed ends of actin filaments, preventing subunit exchange without severing the filaments. CAPZA2 Protein, Human (His-SUMO) is the recombinant human-derived CAPZA2 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The CAPZA2 protein is a member of the F-actin capping protein family and binds to the barbed ends of actin filaments, preventing subunit exchange without severing the filaments. CAPZA2 Protein, Human (His-SUMO) is the recombinant human-derived CAPZA2 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
The CAPZA2 protein, a member of F-actin-capping proteins, binds to the fast-growing ends of actin filaments (barbed end) in a Ca(2+)-independent manner, effectively blocking the exchange of subunits at these ends without severing actin filaments, distinguishing it from other capping proteins like gelsolin and severin. CAPZA2 functions as a heterodimer with an alpha and a beta subunit and is an integral component of the WASH complex, which comprises F-actin-capping protein subunit alpha (CAPZA1, CAPZA2, or CAPZA3), F-actin-capping protein subunit beta (CAPZB), and various WASH complex subunits. Additionally, CAPZA2 interacts with RCSD1/CAPZIP and directly associates with CRACD, with this interaction shown to decrease its binding to actin. The versatile nature of CAPZA2 in modulating actin dynamics highlights its role in regulating cytoskeletal architecture and cellular processes associated with actin filament organization.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His;N-SUMO
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Accession
P47755 (A2-A286)
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Molecular Construction
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N-term
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6*His-SUMO
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CAPZA2 (A2-A286)
Accession # P47755 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
CAPZA2; F-Actin Capping Protein Alpha-2 Subunit; Capping Actin Protein Of Muscle Z-Line Subunit Alpha 2; F-Actin-Capping Protein Subunit Alpha-2; CAPPA2; CapZ Alpha-2; CAPZ; Epididymis Secretory Sperm Binding Protein; Capping Protein (Actin Filament) Musc
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AA Sequence
ADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA
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Predicted Molecular Mass
48.8 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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