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Caspase-14/CASP14 Protein, Human (His)

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Caspase-14 Protein, Human (His) is an approximately 31.0 kDa caspase-14 protein with a His-flag. Caspase-14 is a seemingly non-apoptotic caspase involved in keratinocyte differentiation and cornification.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Caspase-14/CASP14 Protein, Human (His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Caspase-14 Protein, Human (His) is an approximately 31.0 kDa caspase-14 protein with a His-flag. Caspase-14 is a seemingly non-apoptotic caspase involved in keratinocyte differentiation and cornification[3].

Background

Caspase 14 is a member of the caspase family. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin[1].
Caspase-14 is a protease that is mainly expressed in suprabasal epidermal layers and activated during keratinocyte cornification[2].
In contrast to many other caspase family members, murine caspase-14 is not cleaved by granzyme B, caspase-1, caspase-2, caspase-3, caspase-6, caspase-7 or caspase-11, but is weakly processed into p18 and p11 subunits by murine caspase-8[2].
Caspase-14 is a seemingly non-apoptotic caspase involved in keratinocyte differentiation and cornification. Caspase-14 possesses a conserved role in barrier formation through involvement in terminal differentiation and fusion of the cytotrophoblast[3].

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • Accession

    P31944 (S2-Q242)

  • Gene ID
    23581  [NCBI]
  • Molecular Construction
    • N-term
    • CASP14 (S2-Q242)
      Accession # P31944
    • 6*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    CASP14; MGC119078; Caspase 14; MGC119079; Caspase-14; Mini-ICE; Caspase 14, Apoptosis-Related Cysteine Peptidase; CASP-14; Caspase 14, Apoptosis-Related Cysteine Protease; MICE; Apoptosis-Related Cysteine Protease; ARCI12

  • AA Sequence

    SNPRSLEEEKYDMSGARLALILCVTKAREGSEEDLDALEHMFRQLRFESTMKRDPTAEQFQEELEKFQQAIDSREDPVSCAFVVLMAHGREGFLKGEDGEMVKLENLFEALNNKNCQALRAKPKVYIIQACRGEQRDPGETVGGDEIVMVIKDSPQTIPTYTDALHVYSTVEGYIAYRHDQKGSCFIQTLVDVFTKRKGHILELLTEVTRRMAEAELVQEGKARKTNPEIQSTLRKRLYLQ

  • Predicted Molecular Mass

    28.7 kDa

  • Molecular Weight

    Approximately 31 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. S Hu, et al. Caspase-14 is a novel developmentally regulated protease. J Biol Chem [Content Brief]

[2]. M Van de Craen, et al. Identification of a new caspase homologue: caspase-14.Cell Death Differ. 1998 Oct;5(10):838-46. [Content Brief]

[3]. L White, et al. Caspase-14: a new player in cytotrophoblast differentiation.Reprod Biomed Online. 2007 Mar;14(3):300-7. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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