ICAM-2/CD102 Protein, Human (HEK293, His)

ICAM-2/CD102 Protein, Human (HEK293, His) is a transmembrane glycoprotein of the immunoglobulin superfamily.

ICAM-2 (CD102), like the structurally related ICAM-1 (CD54) and ICAM-3 (CD50) proteins is a known ligand for the ß2 family of leukocyte integrins. These include CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), and the dendritic cell-specific, ICAM-grabbing non-integrin (DC-SIGN) protein. ICAM-2 harbours two immunoglobulin domains which are homologues to the first and second immunoglobulin domain of ICAM-1 without any known splice variants. ICAM-2 is uniformly present on all subsets of B lymphocyte lineage cells in bone marrow cell suspensions. High level constitutive expression on vascular endothial cells and less responsiveness than ICAM-1 to inflammatory stimuli are also characteristics of ICAM-2. Recombinant ICAM-2 can co-stimulate T cells in vitro. Although ICAM-2 mediates neutrophil transmigration, ICAM-2 appears capable of mediating CD31/PECAM-1-independent leukocyte transmigration. ICAM-2 may also regulate angiogenesis via several mechanisms including survival, cell migration, and Rac activation^[1][2].

Human

HEK293

C-6*His

P13598 (K25-Q223)

3384  [NCBI]

Extracellular Domain

KVFEVHVRPKKLAVEPKGSLEVNCSTTCNQPEVGGLETSLDKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQ

Approximately 43 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/ccrf-cem-cell.html  [Content Brief]

  [2]. Yoshio Yamashita, et al. Participation of intercellular adhesion molecule-2 (CD102) in B lymphopoiesis. Immunol Lett. 2008 Oct 30;120(1-2):79-86.  [Content Brief]

  [3]. Ruth Lyck, et al. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr Opin Hematol. 2015 Jan;22(1):53-9.  [Content Brief]