CD131 Protein, Human (HEK293, hFc)
The CD131 protein is a cell surface receptor that plays a critical role in the immune response and controls the generation and differentiation of hematopoietic progenitor cells. CD131 Protein, Human (HEK293, hFc) is the recombinant human-derived CD131 protein, expressed by HEK293, with C-hFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CD131 protein is a cell surface receptor that plays a critical role in the immune response and controls the generation and differentiation of hematopoietic progenitor cells. CD131 Protein, Human (HEK293, hFc) is the recombinant human-derived CD131 protein, expressed by HEK293, with C-hFc labeled tag.
Background
CD131, a cell surface receptor, plays a pivotal role in immune response by controlling the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Through the formation of a heterodimeric receptor with various partners such as IL3RA, IL5RA, or CSF2RA, CD131 engages in multiple signaling pathways, including interleukin-3, interleukin-5, and granulocyte-macrophage colony-stimulating factor/CSF2 pathways. In unstimulated conditions, CD131 constitutively interacts with JAK1, and ligand binding leads to JAK1 stimulation, triggering the activation of the JAK-STAT pathway. CD131 forms a heterodimer composed of an alpha and a beta subunit, with the beta subunit being common to the IL3, IL5, and GM-CSF receptors. The GM-CSF receptor complex, involved in signaling, is a dodecamer consisting of two head-to-head hexamers of two alpha, two beta, and two ligand subunits. CD131 further interacts with TMEM102, FCER1G, LYN, and JAK1, contributing to its intricate role in cellular responses.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-hFc
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Accession
P32927-1 (W17-W443)
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Gene ID1439
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Molecular Construction
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N-term
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CD131 (W17-W443)
Accession # P32927-1 -
hFc
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CSF2RB; Beta Common Cytokine Receptor; Prev. IL3RB; CDw131; IL5RB; Interleukin 3 Receptor/Granulocyte-Macrophage Colony Stimulating Factor 3 Receptor, Beta (High Affinity); GM-CSF/IL-3/IL-5 Receptor Common Beta Subunit; Colony-Stimulating Factor-2 Receptor, Beta, Low-Affinity; Cytokine Receptor Common Subunit Beta; GM-CSF/IL-3/IL-5 Receptor Common Beta-Chain; BetaGMR; Alternative Protein CSF2RB; CD131; CD131 Antigen; Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage); SMDP5; Colony Stimulating Factor 2 Receptor Beta Common Subunit; Colony Stimulating Factor 2 Receptor Subunit Beta; Beta-GM-CSF Receptor
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AA Sequence
WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW
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Predicted Molecular Mass
75.1 kDa
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Molecular Weight
Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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