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CD164 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

CD164 is a sialylamucin that may play a key role in the hematopoietic process, promoting CD34(+) cell adhesion while negatively regulating CD34(+)CD38(lo/-) cell proliferation. It regulates cord blood CD133+ cell migration through the CXCL12/CXCR4 axis and is associated with prostate cancer metastasis and bone marrow invasion. CD164 Protein, Human (HEK293, His) is the recombinant human-derived CD164 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD164 Protein, Human (HEK293, His)
  • Experimental Validation Results for CD164 Protein, Human (HEK293, His)
  • CD164 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CD164 is a sialylamucin that may play a key role in the hematopoietic process, promoting CD34(+) cell adhesion while negatively regulating CD34(+)CD38(lo/-) cell proliferation. It regulates cord blood CD133+ cell migration through the CXCL12/CXCR4 axis and is associated with prostate cancer metastasis and bone marrow invasion. CD164 Protein, Human (HEK293, His) is the recombinant human-derived CD164 protein, expressed by HEK293 , with C-His labeled tag.

Background

CD164 is a sialomucin that potentially holds a pivotal role in hematopoiesis, facilitating the adhesion of CD34(+) cells to the stroma while negatively regulating the proliferation of CD34(+)CD38(lo/-) cells. This protein is implicated in the modulation of umbilical cord blood CD133+ cell migration through the CXCL12/CXCR4 axis. Additionally, CD164 is associated with prostate cancer metastasis and the infiltration of cancer cells into the bone marrow. It exerts a positive influence on myogenesis by enhancing CXCR4-dependent cell motility, promoting myoblast migration, and facilitating myoblast fusion into myotubes. The protein exists as a homodimer (isoform 4) and interacts with CXCR4 in these cellular processes.

Verified Bioactivity

Measured by its ability to chemoattract of PC-3 cells. The ED50 for this effect is 1-10 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to chemoattract of PC-3 cells. The ED50 for this effect is 6.165 μg/mL, corresponding to a specific activity is 162.2 U/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q04900-1 (D24-D162)

  • Gene ID
    8763  [NCBI]
  • Molecular Construction
    • N-term
    • CD164 (D24-D162)
      Accession # Q04900-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CD164; Endolyn; CD164 Molecule; DFNA66; MGC-24v; CD164 Antigen, Sialomucin; MUC-24; Deafness, Autosomal Dominant 66; MGC-24; CD164 Molecule, Sialomucin; Multi-Glycosylated Core Protein 24; CD164 Variant C; Sialomucin Core Protein 24; CD164 Antigen

  • AA Sequence

    DKNTTQHPNVTTLAPISNVTSAPVTSLPLVTTPAPETCEGRNSCVSCFNVSVVNTTCFWIECKDESYCSHNSTVSDCQVGNTTDFCSVSTATPVPTANSTAKPTVQPSPSTTSKTVTTSGTTNNTVTPTSQPVRKSTFD

  • Molecular Weight

    Approximately 40-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to chemoattract of PC-3 cells. The ED50 for this effect is 6.165 μg/mL, corresponding to a specific activity is 162.2 U/mg.

  • CD164 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD164 Protein, Human (HEK293, His)
  • Experimental Validation Results for CD164 Protein, Human (HEK293, His)
  • CD164 Protein, Human (HEK293, His) Product Image