Copy Link Print Information

CD39 Protein, Human (sf9, His)

Customer Review

Based on 1 Customer Validation

The CD39 protein is an important regulator in the nervous system, coordinating purinergic neurotransmission by hydrolyzing ATP and other nucleotides. Its enzymatic ability can hydrolyze platelet-activated ADP into AMP, thereby effectively preventing platelet aggregation. CD39 Protein, Human (sf9, His) is the recombinant human-derived CD39 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for CD39 Protein, Human (sf9, His)
  • Experimental Validation Results for CD39 Protein, Human (sf9, His)
  • Experimental Validation Results for CD39 Protein, Human (sf9, His)
  • CD39 Protein, Human (sf9, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The CD39 protein is an important regulator in the nervous system, coordinating purinergic neurotransmission by hydrolyzing ATP and other nucleotides. Its enzymatic ability can hydrolyze platelet-activated ADP into AMP, thereby effectively preventing platelet aggregation. CD39 Protein, Human (sf9, His) is the recombinant human-derived CD39 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

In the nervous system, the CD39 protein serves as a key regulator of purinergic neurotransmission by hydrolyzing ATP and other nucleotides. Its enzymatic activity extends to the prevention of platelet aggregation, as it efficiently hydrolyzes platelet-activating ADP to AMP. Notably, CD39 exhibits equal proficiency in the hydrolysis of both ATP and ADP, emphasizing its versatile role in modulating purinergic signaling pathways and contributing to the intricate regulation of neurotransmission and platelet function.

Verified Bioactivity

1. Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP) and the specific activity is >5000 pmol/min/µg.
2. Loaded Perenostobart (HY-P990068) on AHC2 biosensor, can bind CD39 Protein, Human (sf9, His) with an affinity constant of 3.546E-09 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-His
  • Accession

    P49961 (T38-V478)

  • Gene ID
    953  [NCBI]
  • Molecular Construction
    • N-term
    • CD39 (T38-V478)
      Accession # P49961
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    ENTPD1; ATP Diphosphohydrolase; Prev. CD39; Ecto-ATPase 1; ATPDase; CD39 Antigen; NTPDase-1; Ecto-Apyrase; SPG64; NTPDase1; Nucleoside Triphosphate Diphosphohydrolase 1; ATP-DPH; Lymphoid Cell Activation Antigen; Alternative Protein ENTPD1; Ecto-ATP Dipho

  • AA Sequence

    TQNKALPENVKYGIVLDAGSSHTSLYIYKWPAEKENDTGVVHQVEECRVKGPGISKFVQKVNEIGIYLTDCMERAREVIPRSQHQETPVYLGATAGMRLLRMESEELADRVLDVVERSLSNYPFDFQGARIITGQEEGAYGWITINYLLGKFSQKTRWFSIVPYETNNQETFGALDLGGASTQVTFVPQNQTIESPDNALQFRLYGKDYNVYTHSFLCYGKDQALWQKLAKDIQVASNEILRDPCFHPGYKKVVNVSDLYKTPCTKRFEMTLPFQQFEIQGIGNYQQCHQSILELFNTSYCPYSQCAFNGIFLPPLQGDFGAFSAFYFVMKFLNLTSEKVSQEKVTEMMKKFCAQPWEEIKTSYAGVKEKYLSEYCFSGTYILSLLLQGYHFTADSWEHIHFIGKIQGSDAGWTLGYMLNLTNMIPAEQPLSTPLSHSTYV

  • Predicted Molecular Mass

    51.8 kDa

  • Molecular Weight

    Approximately 57 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.1.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Loaded Perenostobart (HY-P990068) on AHC2 biosensor, can bind CD39 Protein, Human (sf9, His) with an affinity constant of 3.546E-09 M as determined in BLI assay.

  • 1/16

    MedChemExpress Validation
     Loaded Eurestobart (HY-P990038) on ProA biosensor, can bind CD39 Protein, Human (sf9, His) with an affinity constant of 2.503E-09 M as determined in BLI assay.

  • CD39 Protein, Human (sf9, His) Product Image
    1/16
  • Experimental Validation Results for CD39 Protein, Human (sf9, His)
  • Experimental Validation Results for CD39 Protein, Human (sf9, His)
  • Experimental Validation Results for CD39 Protein, Human (sf9, His)
  • CD39 Protein, Human (sf9, His) Product Image