CD3D-CD3E Heterodimer Protein, Mouse (HEK293)

The CD3D protein is a crucial component of the TCR-CD3 complex on T-lymphocytes, essential for adaptive immune responses. Upon TCR activation by APCs, CD3D, along with CD3E, CD3G, and CD3Z, transmits signals through ITAMs, activating downstream pathways. CD3D is vital for proper thymocyte differentiation, ensuring intracellular TCR-CD3 complex assembly. Dysfunctional complexes impair thymocyte differentiation. CD3D also interacts with CD4 and CD8, linking TCR to coreceptors for CD4 or CD8 T-cell activation and positive selection. The CD3D contributes to the intricate network governing T-cell responses. CD3D-CD3E Heterodimer Protein, Mouse (HEK293) is a recombinant protein dimer complex containing mouse-derived CD3D-CD3E Heterodimer protein, expressed by HEK293, with tag free. CD3D-CD3E Heterodimer Protein, Mouse (HEK293), has molecular weight of 45-50 kDa.

The CD3D protein is a crucial component of the TCR-CD3 complex found on the surface of T-lymphocytes, playing a pivotal role in adaptive immune responses. Upon activation of the T-cell receptor (TCR) by antigen-presenting cells (APCs), CD3D, along with CD3E, CD3G, and CD3Z, transmits TCR-mediated signals across the cell membrane. These CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain, which, upon phosphorylation by LCK and FYN kinases, activate downstream signaling pathways. Beyond its role in signal transduction for T-cell activation, CD3D is indispensable in thymocyte differentiation, contributing to proper intracellular TCR-CD3 complex assembly and surface expression. Dysfunction in the TCR-CD3 complex leads to impaired thymocyte differentiation. CD3D further interacts with CD4 and CD8, establishing a functional link between the TCR and coreceptors CD4 and CD8, crucial for the activation and positive selection of CD4 or CD8 T-cells. The TCR-CD3 complex consists of CD3D/CD3E and CD3G/CD3E heterodimers, forming trimers that associate with TCRalpha and TCRbeta. Additionally, the hexamer interacts with CD3Z homodimer to complete the TCR-CD3 complex, wherein TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. This intricate interaction network highlights the multifaceted role of CD3D in orchestrating T-cell responses.

Mouse

HEK293

Tag Free

P04235 (F22-A105)&P22646 (D22-D108)

12500  [NCBI]&12501  [NCBI]

45-50 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.