CD40L/CD154/TRAP Protein, Human (C194W)

CD40L/CD154/TRAP proteins stimulate T cell proliferation and cytokine production, acting as ligands for integrins and CD40 receptors to activate various cell-dependent effects such as B cell activation and NF-κ-B signaling. CD40L/CD154/TRAP Protein, Human (C194W) is the recombinant human-derived CD40L/CD154/TRAP protein, expressed by E. coli , with tag free. The total length of CD40L/CD154/TRAP Protein, Human (C194W) is 149 a.a., with molecular weight of ~16.3 kDa.

CD40L, also known as CD154 or TRAP protein, functions as a cytokine and serves as a ligand for CD40/TNFRSF5, orchestrating a cascade of immunological responses. It plays a pivotal role in T-cell activation, acting as a potent co-stimulator that enhances both T-cell proliferation and cytokine production, specifically IL4 and IL10. Upon cross-linking on T-cells, CD40L generates a costimulatory signal synergizing with TCR/CD3 ligation and CD28 costimulation. Additionally, CD40L induces the activation of NF-kappa-B, triggers the activation of kinases MAPK8 and PAK2 in T-cells, and facilitates tyrosine phosphorylation of CD28 isoform 3. Beyond T-cell modulation, CD40L mediates B-cell proliferation in the absence of co-stimulus and promotes IgE production in the presence of IL4, contributing to immunoglobulin class switching. Furthermore, it serves as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3, and collaborates with the CD40 receptor in the activation of CD40-CD40LG signaling, exerting cell-type-dependent effects such as B-cell activation, NF-kappa-B signaling, and anti-apoptotic signaling.

Measured by its binding ability in a functional ELISA. Immobilized Human CD40 Protein at 5 µg/mL (100µL/well) can bind biotinylated Human CD40L. The ED₅₀ for this effect is 22.31 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human CD40 Protein at 5 µg/mL (100µL/well) can bind biotinylated Human CD40L. The ED₅₀ for this effect is 22.31 ng/mL.

Human

E. coli

Tag Free

P29965 (M113-L261, C194W)

959  [NCBI]

Partial

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Approximately 16 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm solution of PBS, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.