CD46 Protein, Human (HEK293, hFc)

CD46 Protein serves as a cofactor for complement factor I, safeguarding cells against complement-induced harm. It aids in sperm-oocyte fusion and acts as a T-cell costimulatory factor, facilitating the formation of T-regulatory 1 cells. These cells secrete interleukin-10, suppressing immune responses and preventing autoimmunity. Pathogens can exploit CD46's immune regulation abilities to induce an immunosuppressive state in T-cells. CD46 Protein, Human (HEK293, hFc) is the recombinant human-derived CD46 protein, expressed by HEK293, with C-hFc labeled tag. The total length of CD46 Protein, Human (HEK293, hFc) is 294 a.a., with molecular weight of ~70.0 kDa.

CD46 Protein acts as a cofactor for complement factor I, protecting cells from complement-mediated injury by cleaving C3b and C4b on host tissue. It also plays a role in sperm-oocyte fusion during fertilization and acts as a costimulatory factor for T-cells, promoting the differentiation of CD4+ cells into T-regulatory 1 cells. These T-regulatory 1 cells suppress immune responses by secreting interleukin-10, thus preventing autoimmunity. In the context of microbial infection, certain viral and bacterial pathogens exploit CD46's immune regulation properties by binding to it and inducing an immunosuppressive phenotype in T-cells.

Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/mL can bind Anti-CD46 rabbit monoclonal antibody, the EC₅₀ of human CD46 protein is 0.8-1.38 ng/mL.

Human

HEK293

C-hFc

P15529 (C35-Y328)

4179  [NCBI]

CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY

Approximately 70.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.