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  5. CD5L Protein, Human (HEK293, His)

CD5L Protein, Human (HEK293, His)

Cat. No.: HY-P72718
COA Handling Instructions

CD5L protein regulates lipid metabolism and lipolysis by promoting fatty acid endocytosis into adipocytes via the CD36 receptor. This process, crucial for maintaining lipid homeostasis, leads to the degradation of triacylglycerols into glycerol and free fatty acids. CD5L Protein, Human (HEK293, His) is the recombinant human-derived CD5L protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD5L Protein, Human (HEK293, His) is 328 a.a., with molecular weight of 38-50 kDa.

For research use only. We do not sell to patients.

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Description

CD5L protein regulates lipid metabolism and lipolysis by promoting fatty acid endocytosis into adipocytes via the CD36 receptor. This process, crucial for maintaining lipid homeostasis, leads to the degradation of triacylglycerols into glycerol and free fatty acids. CD5L Protein, Human (HEK293, His) is the recombinant human-derived CD5L protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD5L Protein, Human (HEK293, His) is 328 a.a., with molecular weight of 38-50 kDa.

Background

CD5L is a protein that plays multiple roles in various biological processes. One of its main functions is to regulate lipid metabolism and lipolysis. It does this by promoting the endocytosis of fatty acids into adipocytes via the CD36 receptor, which leads to the degradation of triacylglycerols into glycerol and free fatty acids. This process is important in maintaining proper lipid homeostasis in the body.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

O43866 (S20-G347)

Gene ID

922  [NCBI]

Molecular Construction
N-term
CD5L (S20-G347)
Accession # O43866
6*His
C-term
Synonyms
CD5 antigen-like; mAIM; SP-alpha; Cd5l; AIM; API6
AA Sequence

SPSGVRLVGGLHRCEGRVEVEQKGQWGTVCDDGWDIKDVAVLCRELGCGAASGTPSGILYEPPAEKEQKVLIQSVSCTGTEDTLAQCEQEEVYDCSHDEDAGASCENPESSFSPVPEGVRLADGPGHCKGRVEVKHQNQWYTVCQTGWSLRAAKVVCRQLGCGRAVLTQKRCNKHAYGRKPIWLSQMSCSGREATLQDCPSGPWGKNTCNHDEDTWVECEDPFDLRLVGGDNLCSGRLEVLHKGVWGSVCDDNWGEKEDQVVCKQLGCGKSLSPSFRDRKCYGPGVGRIWLDNVRCSGEEQSLEQCQHRFWGFHDCTHQEDVAVICSG

Molecular Weight

38-50 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD5L Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD5L Protein, Human (HEK293, His)
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HY-P72718
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