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  5. CD69
  6. CD69 Protein, Mouse (HEK293, His)

CD69 Protein, a signal transmitting receptor, mediates lymphocyte proliferation in NK cells, platelets, and lymphocytes. It facilitates homodimer formation through disulfide-linkage. CD69 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD69 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

CD69 Protein, a signal transmitting receptor, mediates lymphocyte proliferation in NK cells, platelets, and lymphocytes. It facilitates homodimer formation through disulfide-linkage. CD69 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD69 protein, expressed by HEK293 , with C-His labeled tag.

Background

CD69 Protein, a signal transmitting receptor, is involved in lymphocyte proliferation and functions in lymphocytes, natural killer (NK) cells, and platelets. It forms a homodimer through disulfide-linkage.

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD69 is coated at 1 µg/mL (100 µL/well) can blind Recombinant Mouse Galectin-1. The ED50 for this effect is 50.65 ng/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD69 is coated at 1 µg/mL (100 µL/well) can blind Recombinant Mouse Galectin-1 .The ED50 for this effect is 50.65 ng/mL.
Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

P37217 (N62-R199)

Gene ID
Molecular Construction
N-term
CD69 (N62-R199)
Accession # P37217
His
C-term
Protein Length

Extracellular Domain

Synonyms
Early activation antigen CD69; AIM; MLR-3; CD69; CLEC2C
AA Sequence

NVGKYNCPGLYEKLESSDHHVATCKNEWISYKRTCYFFSTTTKSWALAQRSCSEDAATLAVIDSEKDMTFLKRYSGELEHWIGLKNEANQTWKWANGKEFNSWFNLTGSGRCVSVNHKNVTAVDCEANFHWVCSKPSR

Molecular Weight

Approximately 24-32 kDa due to the glycosylation.

Glycosylation
Yes
Purity
  • ≥ 85%, as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD69 Protein, Mouse (HEK293, His)
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HY-P74274
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