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  5. CD84/SLAMF5 Protein, Human (Biotinylated, HEK293, His-Avi)

CD84/SLAMF5 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P72414
Handling Instructions

The CD84/SLAMF5 protein, a self-ligand receptor in the SLAM family, modulates diverse immune cell activities through homo- or heterotypic cell-cell interactions. It finely tunes its functions based on the presence or absence of small cytoplasmic adapter proteins, including SH2D1A/SAP and/or SH2D1B/EAT-2. CD84/SLAMF5 mediates NK cell cytotoxicity dependent on SH2D1A and SH2D1B, enhances T-cell proliferative responses, and amplifies IFNG secretion. It is crucial for prolonged T-cell:B-cell contact, germinal center formation, and maintaining B-cell tolerance. In mast cells, it negatively regulates immunoglobulin epsilon receptor signaling, and in macrophages, it positively regulates LPS-induced MAPK phosphorylation and NF-kappaB activation. Additionally, CD84/SLAMF5 positively regulates macroautophagy in dendritic cells and forms homodimers, interacting with CD48 molecules and various signaling proteins, including SH2D1A/SAP, SH2D1B/EAT-2, PTPN6/SHP-1, PTPN11/SHP-2, and INPP5D/SHIP1. CD84/SLAMF5 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD84/SLAMF5 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag. The total length of CD84/SLAMF5 Protein, Human (Biotinylated, HEK293, His-Avi) is 199 a.a., with molecular weight of 40-50 kDa.

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Description

The CD84/SLAMF5 protein, a self-ligand receptor in the SLAM family, modulates diverse immune cell activities through homo- or heterotypic cell-cell interactions. It finely tunes its functions based on the presence or absence of small cytoplasmic adapter proteins, including SH2D1A/SAP and/or SH2D1B/EAT-2. CD84/SLAMF5 mediates NK cell cytotoxicity dependent on SH2D1A and SH2D1B, enhances T-cell proliferative responses, and amplifies IFNG secretion. It is crucial for prolonged T-cell:B-cell contact, germinal center formation, and maintaining B-cell tolerance. In mast cells, it negatively regulates immunoglobulin epsilon receptor signaling, and in macrophages, it positively regulates LPS-induced MAPK phosphorylation and NF-kappaB activation. Additionally, CD84/SLAMF5 positively regulates macroautophagy in dendritic cells and forms homodimers, interacting with CD48 molecules and various signaling proteins, including SH2D1A/SAP, SH2D1B/EAT-2, PTPN6/SHP-1, PTPN11/SHP-2, and INPP5D/SHIP1. CD84/SLAMF5 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD84/SLAMF5 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag. The total length of CD84/SLAMF5 Protein, Human (Biotinylated, HEK293, His-Avi) is 199 a.a., with molecular weight of 40-50 kDa.

Background

The CD84/SLAMF5 protein serves as a self-ligand receptor within the signaling lymphocytic activation molecule (SLAM) family, engaging in homo- or heterotypic cell-cell interactions that modulate the activation and differentiation of diverse immune cells, contributing to the intricate regulation and interconnection of both innate and adaptive immune responses. The protein's activities are finely tuned by the presence or absence of small cytoplasmic adapter proteins, including SH2D1A/SAP and/or SH2D1B/EAT-2. CD84/SLAMF5 can mediate natural killer (NK) cell cytotoxicity, dependent on both SH2D1A and SH2D1B. It enhances proliferative responses of activated T-cells, and SH2D1A/SAP appears not to be required for this process. Homophilic interactions amplify interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. The protein may serve as a marker for hematopoietic progenitor cells. Essential for prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation, CD84/SLAMF5 in germinal centers contributes to maintaining B-cell tolerance and preventing autoimmunity. In mast cells, it negatively regulates high-affinity immunoglobulin epsilon receptor signaling, independently of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1. In macrophages, it positively regulates LPS-induced MAPK phosphorylation and NF-kappaB activation, modulating LPS-induced cytokine secretion. Additionally, CD84/SLAMF5 positively regulates macroautophagy in primary dendritic cells through the stabilization of IRF8 and inhibits TRIM21-mediated proteasomal degradation of IRF8. The protein forms homodimers via its extracellular domain and interacts with CD48 molecules from other cells in a head-to-tail dimeric arrangement. It also interacts with SH2 domain-containing proteins, including SH2D1A/SAP and SH2D1B/EAT-2, and tyrosine-protein phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 via its phosphorylated cytoplasmic domain, with the latter interaction blocked by SH2D1A. CD84/SLAMF5 further interacts with INPP5D/SHIP1 through its phosphorylated ITSM domains.

Species

Human

Source

HEK293

Tag

C-Avi;C-6*His

Accession

Q9UIB8-1 (K22-R220)

Gene ID
Molecular Construction
N-term
CD84 (K22-R220)
Accession # Q9UIB8-1
6*His-Avi
C-term
Synonyms
SLAM family member 5; Cell surface antigen MAX.3; Hly9-beta; Signaling lymphocytic activation molecule 5; CD84
AA Sequence

KDSEIFTVNGILGESVTFPVNIQEPRQVKIIAWTSKTSVAYVTPGDSETAPVVTVTHRNYYERIHALGPNYNLVISDLRMEDAGDYKADINTQADPYTTTKRYNLQIYRRLGKPKITQSLMASVNSTCNVTLTCSVEKEEKNVTYNWSPLGEEGNVLQIFQTPEDQELTYTCTAQNPVSNNSDSISARQLCADIAMGFR

Molecular Weight

40-50 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CD84/SLAMF5 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD84/SLAMF5 Protein, Human (Biotinylated, HEK293, His-Avi)
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HY-P72414
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