CD96 Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
CD96 promotes adhesive interactions between activated T cells and NK cells in the late stages of the immune response. It promotes the adhesion between NK cells and target cells by interacting with PVR on target cells. CD96 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD96 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CD96 promotes adhesive interactions between activated T cells and NK cells in the late stages of the immune response. It promotes the adhesion between NK cells and target cells by interacting with PVR on target cells. CD96 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD96 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background
CD96 protein appears to play a role in adhesive interactions during the late phase of the immune response, potentially involving activated T and NK cells. It facilitates NK cell-target adhesion by interacting with PVR present on target cells. CD96 may function in a phase after T and NK cells have penetrated the endothelium using integrins and selectins, actively engaging with diseased cells and navigating within areas of inflammation. Structurally, CD96 forms homodimers through disulfide linkages and interacts specifically with PVR, suggesting its involvement in cell-cell interactions crucial for immune responses during inflammation and infection.
Verified Bioactivity
Immobilized Biotinylated Human CD96, His-Avi Tag at 2μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-CD96 Antibody, hFc Tag with the EC50 of 16.4ng/ml determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
P40200-2 (V22-M503)
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Molecular Construction
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N-term
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CD96 (V22-M503)
Accession # P40200-2 -
8*His-Avi
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C-term
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Protein Length
Partial Extracellular Domain
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Conjugation
Biotin
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Synonyms
CD96; TACTILE; CD96 Molecule; T Cell Activation, Increased Late Expression; T-Cell Surface Protein Tactile; CD96 Antigen; T Cell-Activated Increased Late Expression Protein; Alternative Protein CD96; Cell Surface Antigen CD96
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AA Sequence
VWEKTVNTEENVYATLGSDVNLTCQTQTVGFFVQMQWSKVTNKIDLIAVYHPQYGFYCAYGRPCESLVTFTETPENGSKWTLHLRNMSCSVSGRYECMLVLYPEGIQTKIYNLLIQTHVTADEWNSNHTIEIEINQTLEIPCFQNSSSKISSEFTYAWSVEDNGTQETLISQNHLISNSTLLKDRVKLGTDYRLHLSPVQIFDDGRKFSCHIRVGPNKILRSSTTVKVFAKPEIPVIVENNSTDVLVERRFTCLLKNVFPKANITWFIDGSFLHDEKEGIYITNEERKGKDGFLELKSVLTRVHSNKPAQSDNLTIWCMALSPVPGNKVWNISSEKITFLLGSEISSTDPPLSVTESTLDTQPSPASSVSPARYPATSSVTLVDVSALRPNTTPQPSNSSMTTRGFNYPWTSSGTDTKKSVSRIPSETYSSSPSGAGSTLHDNVFTSTARAFSEVPTTANGSTKTNHVHITGIVVNKPKDGM
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Predicted Molecular Mass
59.1 kDa
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Molecular Weight
Approximately 90-140 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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