1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2) Serine/Threonine Kinase Proteins
  4. Cyclin-Dependent Kinases (CDKs)
  5. Cyclin Dependent Kinase 1 (CDK1)
  6. CDK1 Protein, Mouse (sf9, His-GST)

CDK1 Protein, Mouse (sf9, His-GST)

Cat. No.: HY-P75669
Handling Instructions

The CDK1 protein centrally controls the eukaryotic cell cycle, coordinating the G2-M transition and mitotic events. It interacts with a variety of cell cycle proteins, phosphorylates a variety of substrates, and affects cell cycle regulation, DNA repair, and apoptosis. CDK1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived CDK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of CDK1 Protein, Mouse (sf9, His-GST) is 297 a.a., with molecular weight of ~57 kDa.

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Description

The CDK1 protein centrally controls the eukaryotic cell cycle, coordinating the G2-M transition and mitotic events. It interacts with a variety of cell cycle proteins, phosphorylates a variety of substrates, and affects cell cycle regulation, DNA repair, and apoptosis. CDK1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived CDK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of CDK1 Protein, Mouse (sf9, His-GST) is 297 a.a., with molecular weight of ~57 kDa.

Background

CDK1 Protein plays a central role in controlling the eukaryotic cell cycle by modulating both the centrosome cycle and mitotic onset. It facilitates the G2-M transition by associating with multiple interphase cyclins and phosphorylates an extensive array of substrates involved in diverse cellular processes, including cell cycle regulation, DNA repair, and apoptosis. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes, triggering centrosome separation, Golgi dynamics, nuclear envelope breakdown, and chromosome condensation. Upon successful alignment of chromosomes at the metaphase plate, CDK1 activity is turned off by WEE1- and PKMYT1-mediated phosphorylation, allowing sister chromatid separation, chromosome decondensation, nuclear envelope reformation, and cytokinesis. CDK1 also plays a role in cellular responses to DNA damage, as it is inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation, contributing to the G2 checkpoint. Reactivation after DNA repair occurs through WIP1-dependent signaling, leading to CDC25A/B/C-mediated dephosphorylation and the resumption of cell cycle progression. Additionally, CDK1 phosphorylates various substrates involved in microtubule dynamics, spindle formation, and cell survival, highlighting its multifaceted regulatory role in orchestrating essential cellular events.

Species

Mouse

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

P11440 (M1-M297)

Gene ID
Molecular Construction
N-term
His-GST
CDK1 (M1-M297)
Accession # P11440
C-term
Synonyms
Cyclin-dependent kinase 1; CDK1; p34 protein kinase; CDC2; CDC28A; CDKN1; P34CDC2
Molecular Weight

Approximately 57 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CDK1 Protein, Mouse (sf9, His-GST)
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HY-P75669
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