CHST3 Protein, Mouse (HEK293, His)

The CHST3 protein utilizes PAPS to keyly catalyze the transfer of sulfate to position 6 of GalNAc in chondroitin, the major proteoglycan in cartilage. CHST3 also sulfonates Gal residues in keratan sulfate and sialic acid LacNAc oligosaccharides with less efficiency. CHST3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHST3 protein, expressed by HEK293 , with N-His labeled tag.

CHST3 protein, a sulfotransferase utilizing 3'-phospho-5'-adenylyl sulfate (PAPS) as its sulfonate donor, serves as a crucial enzyme catalyzing the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin, the predominant proteoglycan in cartilage and widely distributed on cell surfaces and extracellular matrices. While exhibiting a lower efficiency, CHST3 can also sulfonate the Gal residues of keratan sulfate, another glycosaminoglycan, and catalyze the sulfation of Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. Beyond its glycan-modifying activities, CHST3 may play a role in maintaining naive T-lymphocytes in the spleen. The versatile sulfation capabilities of CHST3 underscore its significance in modulating the sulfation patterns of key glycosaminoglycans, contributing to the structural diversity of the extracellular matrix and potentially influencing immune cell dynamics in specific tissues.

Measured by its ability to transfer sulfate from PAPS to chondroitin sulfate. The specific activity is 3316 pmol/min/μg that incubate at 37 ºC for 20 minutes.

Mouse

HEK293

N-10*His

O88199 (E39-T472)

53374  [NCBI]

Lumenal Domain

EKENKIISRVSDKLKQIPHFVADANSTDPALLLSENASLLSLSELDSTFSHLRSRLHNLSLQLGVEPAMESQEAGAEKPSQQAGAGTRRHVLLMATTRTGSSFVGEFFNQQGNIFYLFEPLWHIERTVFFQQRGASAAGSALVYRDVLKQLLLCDLYVLEPFISPPPEDHLTQFLFRRGSSRSLCEDPVCTPFVKKVFEKYHCRNRRCGPLNVTLAGEACRRKDHVALKAVRIRQLEFLQPLVEDPRLDLRVIQLVRDPRAVLASRIVAFAGKYENWKKWLSEGQDQLSEDEVQRLRGNCESIRLSAELGLRQPAWLRGRYMLVRYEDVARRPLQKAREMYSFAGIPLTPQVEDWIQKNTQATRDSSDVYSTQKNSSEQFEKWRFSMPFKLAQVVQAACGPTMHLFGYKLARDAASLTNRSISLLEERGTFWVT

Approximately 55-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.