CHST3 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
The CHST3 protein utilizes PAPS to keyly catalyze the transfer of sulfate to position 6 of GalNAc in chondroitin, the major proteoglycan in cartilage. CHST3 also sulfonates Gal residues in keratan sulfate and sialic acid LacNAc oligosaccharides with less efficiency. CHST3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHST3 protein, expressed by HEK293 , with N-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The CHST3 protein utilizes PAPS to keyly catalyze the transfer of sulfate to position 6 of GalNAc in chondroitin, the major proteoglycan in cartilage. CHST3 also sulfonates Gal residues in keratan sulfate and sialic acid LacNAc oligosaccharides with less efficiency. CHST3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHST3 protein, expressed by HEK293 , with N-His labeled tag.
CHST3 protein, a sulfotransferase utilizing 3'-phospho-5'-adenylyl sulfate (PAPS) as its sulfonate donor, serves as a crucial enzyme catalyzing the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin, the predominant proteoglycan in cartilage and widely distributed on cell surfaces and extracellular matrices. While exhibiting a lower efficiency, CHST3 can also sulfonate the Gal residues of keratan sulfate, another glycosaminoglycan, and catalyze the sulfation of Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. Beyond its glycan-modifying activities, CHST3 may play a role in maintaining naive T-lymphocytes in the spleen. The versatile sulfation capabilities of CHST3 underscore its significance in modulating the sulfation patterns of key glycosaminoglycans, contributing to the structural diversity of the extracellular matrix and potentially influencing immune cell dynamics in specific tissues.
Measured by its ability to transfer sulfate from PAPS to chondroitin sulfate. The specific activity is 3316 pmol/min/μg that incubate at 37 ºC for 20 minutes.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag N-10*His
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Accession
O88199 (E39-T472)
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Molecular Construction
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N-term
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His
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CHST3 (E39-T472)
Accession # O88199 -
C-term
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Protein Length
Lumenal Domain
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Synonyms
CHST3; Chondroitin 6-O-Sulfotransferase 1; Carbohydrate Sulfotransferase 3; Chondroitin 6 Sulfotransferase 1; C6ST; C6ST-1; C6ST1; GST-0; Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 0; Chondroitin 6-Sulfotransferase; Carbohydrat
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AA Sequence
EKENKIISRVSDKLKQIPHFVADANSTDPALLLSENASLLSLSELDSTFSHLRSRLHNLSLQLGVEPAMESQEAGAEKPSQQAGAGTRRHVLLMATTRTGSSFVGEFFNQQGNIFYLFEPLWHIERTVFFQQRGASAAGSALVYRDVLKQLLLCDLYVLEPFISPPPEDHLTQFLFRRGSSRSLCEDPVCTPFVKKVFEKYHCRNRRCGPLNVTLAGEACRRKDHVALKAVRIRQLEFLQPLVEDPRLDLRVIQLVRDPRAVLASRIVAFAGKYENWKKWLSEGQDQLSEDEVQRLRGNCESIRLSAELGLRQPAWLRGRYMLVRYEDVARRPLQKAREMYSFAGIPLTPQVEDWIQKNTQATRDSSDVYSTQKNSSEQFEKWRFSMPFKLAQVVQAACGPTMHLFGYKLARDAASLTNRSISLLEERGTFWVT
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Molecular Weight
Approximately 55-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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