1. Recombinant Proteins
  2. Complement System
  3. Complement Regulatory Proteins
  4. Factor H
  5. Complement factor H/CFH Protein, Mouse (HEK293, His)

Complement factor H/CFH Protein, Mouse (HEK293, His)

Cat. No.: HY-P7830
COA Handling Instructions

Complement factor H (CFH) lacks essential conserved residue(s) for feature annotation propagation. Complement factor H/CFH Protein, Mouse (HEK293, His) is the recombinant mouse-derived Complement factor H/CFH protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Complement factor H/CFH Protein, Mouse (HEK293, His) is 378 a.a., with molecular weight of 60-75 kDa.

For research use only. We do not sell to patients.

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Description

Complement factor H (CFH) lacks essential conserved residue(s) for feature annotation propagation. Complement factor H/CFH Protein, Mouse (HEK293, His) is the recombinant mouse-derived Complement factor H/CFH protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Complement factor H/CFH Protein, Mouse (HEK293, His) is 378 a.a., with molecular weight of 60-75 kDa.

Background

Complement factor H (CFH) exhibits the absence of conserved residue(s) crucial for the propagation of feature annotation.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

E9Q8I0 (S875-V1252)

Gene ID

12628  [NCBI]

Molecular Construction
N-term
CFH (S875-V1252)
Accession # E9Q8I0
6*His
C-term
Synonyms
rMuComplement factor H/CFH, His; Complement factor H; Protein beta-1-H; CFH
AA Sequence

SLPRCIEKIPCSQPPTIEHGSINLPRSSEERRDSIESSSHEHGTTFSYVCDDGFRIPEENRITCYMGKWSTPPRCVGLPCGPPPSIPLGTVSLELESYQHGEEVTYHCSTGFGIDGPAFIICEGGKWSDPPKCIKTDCDVLPTVKNAIIRGKSKKSYRTGEQVTFRCQSPYQMNGSDTVTCVNSRWIGQPVCKDNSCVDPPHVPNATIVTRTKNKYLHGDRVRYECNKPLELFGQVEVMCENGIWTEKPKCRDSTGKCGPPPPIDNGDITSLSLPVYEPLSSVEYQCQKYYLLKGKKTITCRNGKWSEPPTCLHACVIPENIMESHNIILKWRHTEKIYSHSGEDIEFGCKYGYYKARDSPPFRTKCINGTINYPTCV

Molecular Weight

60-75 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Complement factor H/CFH Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Complement factor H/CFH Protein, Mouse (HEK293, His)
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HY-P7830
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