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CREG1/CREG Protein, Human (HEK293, His)

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Based on 1 Customer Validation

CREG1/CREG proteins may contribute to the transcriptional control of cell growth and differentiation and play a key role in counteracting cellular transformation induced by the adenovirus E1A protein. Its activity depends on interaction with IGF2R, functioning as a glycosylation-dependent homodimer. CREG1/CREG Protein, Human (HEK293, His) is the recombinant human-derived CREG1/CREG protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CREG1/CREG Protein, Human (HEK293, His)
  • Experimental Validation Results for CREG1/CREG Protein, Human (HEK293, His)
  • CREG1/CREG Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CREG1/CREG proteins may contribute to the transcriptional control of cell growth and differentiation and play a key role in counteracting cellular transformation induced by the adenovirus E1A protein. Its activity depends on interaction with IGF2R, functioning as a glycosylation-dependent homodimer. CREG1/CREG Protein, Human (HEK293, His) is the recombinant human-derived CREG1/CREG protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The CREG1/CREG protein emerges as a potential contributor to the transcriptional control of cell growth and differentiation. It plays a pivotal role in antagonizing transcriptional activation and countering cellular transformation induced by the adenovirus E1A protein. Moreover, the transcriptional control activity related to cell growth is contingent on the interaction between CREG1/CREG and IGF2R. Operating as a homodimer, CREG1/CREG also exhibits an interaction with IGF2R that is glycosylation-dependent. These findings suggest a multifaceted role for CREG1/CREG in regulating cellular processes, shedding light on its involvement in transcriptional control mechanisms and its interactions with key cellular components like IGF2R. A more in-depth exploration of these interactions may provide valuable insights into the nuanced molecular mechanisms governing cell growth and differentiation.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized IGF2R at 2 μg/mL (100μL/well) can bind Biotinylated Human CREG Protein. The ED50 for this effect is 9.563 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized IGF2R at 2 μg/mL (100 μL/well) can bind Biotinylated Human CREG Protein. The ED50 for this effect is 9.563 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    O75629 (R32-Q220)

  • Gene ID
    8804  [NCBI]
  • Molecular Construction
    • N-term
    • CREG1 (R32-Q220)
      Accession # O75629
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CREG1; Cellular Repressor Of E1A-Stimulated Genes 1, Isoform CRA_a; Prev. CREG; Cellular Repressor Of E1A-Stimulated Genes; Protein CREG1; Cellular Repressor Of E1A Stimulated Genes 1; Cellular Repressor Of E1A-Stimulated Genes 1

  • AA Sequence

    RGGRDHGDWDEASRLPPLPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLSPLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAKHSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ

  • Predicted Molecular Mass

    21.9 kDa

  • Molecular Weight

    Approximately 20-38 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized IGF2R at 2 μg/mL (100 μL/well) can bind Biotinylated Human CREG Protein. The ED50 for this effect is 9.563 ng/mL.

  • CREG1/CREG Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CREG1/CREG Protein, Human (HEK293, His)
  • Experimental Validation Results for CREG1/CREG Protein, Human (HEK293, His)
  • CREG1/CREG Protein, Human (HEK293, His) Product Image