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CRELD1 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

CRELD1 Protein, acting as a protein disulfide isomerase, aids in the correct folding and assembly of proteins, crucial for cellular homeostasis. It also contributes to the proper localization of acetylcholine receptors (AChRs) to the plasma membrane, emphasizing its significance in maintaining protein structure and membrane receptor distribution. CRELD1 Protein, Human (HEK293, His) is the recombinant human-derived CRELD1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CRELD1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CRELD1 Protein, Human (HEK293, His)
  • CRELD1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

CRELD1 Protein, acting as a protein disulfide isomerase, aids in the correct folding and assembly of proteins, crucial for cellular homeostasis. It also contributes to the proper localization of acetylcholine receptors (AChRs) to the plasma membrane, emphasizing its significance in maintaining protein structure and membrane receptor distribution. CRELD1 Protein, Human (HEK293, His) is the recombinant human-derived CRELD1 protein, expressed by HEK293 , with C-His labeled tag.

Background

CRELD1 protein, functioning as a protein disulfide isomerase, plays a role in cellular processes related to the correct folding and assembly of proteins. In addition, it contributes to the proper localization of acetylcholine receptors (AChRs) to the plasma membrane. The involvement of CRELD1 in these molecular mechanisms underscores its significance in cellular homeostasis, particularly in the context of protein structure and membrane receptor distribution.

Verified Bioactivity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.5052 μg/mL, corresponding to a specific activity is 1979.022 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.5052 μg/mL, corresponding to a specific activity is 1979.022 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q96HD1-1 (Q30-E362)

  • Gene ID
    78987  [NCBI]
  • Molecular Construction
    • N-term
    • CRELD1 (Q30-E362)
      Accession # Q96HD1-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CRELD1; Cysteine-Rich With EGF-Like Domain Protein 1; Prev. AVSD2; Atrioventricular Septal Defect 2; Cysteine Rich With EGF Like Domains 1; Protein Disulfide-Isomerase; CIRRIN; JELANS; CRELD Disulfide Isomerase 1; Protein Disulfide Isomerase CRELD1

  • AA Sequence

    QPSPPPQSSPPPQPHPCHTCRGLVDSFNKGLERTIRDNFGGGNTAWEEENLSKYKDSETRLVEVLEGVCSKSDFECHRLLELSEELVESWWFHKQQEAPDLFQWLCSDSLKLCCPAGTFGPSCLPCPGGTERPCGGYGQCEGEGTRGGSGHCDCQAGYGGEACGQCGLGYFEAERNASHLVCSACFGPCARCSGPEESNCLQCKKGWALHHLKCVDIDECGTEGANCGADQFCVNTEGSYECRDCAKACLGCMGAGPGRCKKCSPGYQQVGSKCLDVDECETEVCPGENKQCENTEGGYRCICAEGYKQMEGICVKEQIPESAGFFSEMTEDE

  • Molecular Weight

    Approximately 51 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.5052 μg/mL, corresponding to a specific activity is 1979.022 units/mg.

  • CRELD1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CRELD1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CRELD1 Protein, Human (HEK293, His)
  • CRELD1 Protein, Human (HEK293, His) Product Image