CSRP3 Protein, Human (HEK293, His)

CSRP3 is a positive regulator of myogenesis, acting as a cofactor for myogenic bHLH transcription factors such as MYOD1, and likely MYOG and MYF6. It enhances the DNA-binding activity of the MYOD1:TCF3 isoform E47 complex and may promote the formation of a functional MYOD1:TCF3 isoform E47:MEF2A complex involved in myogenesis (By similarity). In cardiomyocytes, CSRP3 plays a crucial and specific role in organizing cytosolic structures, potentially sensing mechanical stretch, and functioning as a scaffold protein to facilitate the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line and required for stress-induced calcineurin-NFAT activation (By similarity). The role of CSRP3 in regulating cytoskeleton dynamics via interaction with CFL2 is controversially reported: it can enhance CFL2-mediated F-actin depolymerization depending on the CSRP3:CFL2 molecular ratio, but also reduces the ability of CLF1 and CFL2 to promote actin depolymerization. CSRP3 may maintain muscle cell integrity through an actin-based mechanism, directly binding to actin filaments, cross-linking them into non-polar bundles, and protecting them from dilution- and cofilin-mediated depolymerization (a function potentially involving self-association). In vitro, CSRP3 inhibits PKC/PRKCA activity and is proposed to modulate cardiac stress signaling by suppressing excessive PKC/PRKCA signaling (By similarity).