DDAH1 Protein, Human (His, Myc)
Based on 1 Customer Validation
DDAH1 protein regulates nitric oxide generation by hydrolyzing N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA), inhibitors of nitric oxide synthase (NOS). This enzymatic activity modulates nitric oxide levels, influencing diverse physiological processes governed by this signaling molecule. DDAH1 Protein, Human (His, Myc) is the recombinant human-derived DDAH1 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
DDAH1 protein regulates nitric oxide generation by hydrolyzing N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA), inhibitors of nitric oxide synthase (NOS). This enzymatic activity modulates nitric oxide levels, influencing diverse physiological processes governed by this signaling molecule. DDAH1 Protein, Human (His, Myc) is the recombinant human-derived DDAH1 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.
DDAH1 protein plays a crucial role in the regulation of nitric oxide generation by hydrolyzing N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA), which act as inhibitors of nitric oxide synthase (NOS). Through this enzymatic activity, DDAH1 contributes to the modulation of nitric oxide levels, impacting various physiological processes influenced by this signaling molecule.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-10*His;C-Myc
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Accession
O94760-1 (A2-S285)
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Molecular Construction
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N-term
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10*His
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DDAH1 (A2-S285)
Accession # O94760-1 -
Myc
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C-term
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Protein Length
Full Length of Isoform 1
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Synonyms
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; EC:3.5.3.18; DDAH-1; Dimethylarginine dimethylaminohydrolase 1; DDAHI; Dimethylargininase-1; DDAH; DDAH1
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AA Sequence
AGLGHPAAFGRATHAVVRALPESLGQHALRSAKGEEVDVARAERQHQLYVGVLGSKLGLQVVELPADESLPDCVFVEDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLTCCSVLINKKVDS
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Predicted Molecular Mass
38.4 kDa
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Molecular Weight
Approximately 43 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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