1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. DDAH1 Protein, Human (His, Myc)

DDAH1 protein regulates nitric oxide generation by hydrolyzing N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA), inhibitors of nitric oxide synthase (NOS). This enzymatic activity modulates nitric oxide levels, influencing diverse physiological processes governed by this signaling molecule. DDAH1 Protein, Human (His, Myc) is the recombinant human-derived DDAH1 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.

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Description

DDAH1 protein regulates nitric oxide generation by hydrolyzing N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA), inhibitors of nitric oxide synthase (NOS). This enzymatic activity modulates nitric oxide levels, influencing diverse physiological processes governed by this signaling molecule. DDAH1 Protein, Human (His, Myc) is the recombinant human-derived DDAH1 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.

Background

DDAH1 protein plays a crucial role in the regulation of nitric oxide generation by hydrolyzing N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA), which act as inhibitors of nitric oxide synthase (NOS). Through this enzymatic activity, DDAH1 contributes to the modulation of nitric oxide levels, impacting various physiological processes influenced by this signaling molecule.

Species

Human

Source

E. coli

Tag

N-10*His;C-Myc

Accession

O94760-1 (A2-S285)

Gene ID
Molecular Construction
N-term
10*His
DDAH1 (A2-S285)
Accession # O94760-1
Myc
C-term
Protein Length

Full Length of Isoform 1

Synonyms
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; DDAH1; DDAH-1; Dimethylarginine dimethylaminohydrolase 1; DDAHI; Dimethylargininase-1
AA Sequence

AGLGHPAAFGRATHAVVRALPESLGQHALRSAKGEEVDVARAERQHQLYVGVLGSKLGLQVVELPADESLPDCVFVEDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLTCCSVLINKKVDS

Predicted Molecular Mass
38.4 kDa
Molecular Weight

Approximately 43 kDa

Purity

≥ 90%, as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

DDAH1 Protein, Human (His, Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
DDAH1 Protein, Human (His, Myc)
Cat. No.:
HY-P79232
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