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DDX5 Protein, Human (His, Myc)

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Based on 1 publication(s) in Google Scholar

DDX5 Protein, Human (His, Myc) is the recombinant human-derived DDX5, expressed by E. coli , with N-10*His, C-Myc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DDX5 Protein, Human (His, Myc)
  • Experimental Validation Results for DDX5 Protein, Human (His, Myc)
  • DDX5 Protein, Human (His, Myc) Product Image
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[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

DDX5 Protein, Human (His, Myc) is the recombinant human-derived DDX5, expressed by E. coli , with N-10*His, C-Myc labeled tag.

Background

DDX5 is an RNA helicase involved in the alternative regulation of pre-mRNA splicing, where its helicase activity promotes tau exon 10 inclusion in an RBM4-dependent manner. It binds to the stem-loop region downstream of exon 10 in tau pre-mRNA, with ATP hydrolysis rates highly stimulated by single-stranded RNA. Beyond splicing, DDX5 participates in transcription regulation (independent of helicase activity), acting as a coactivator for androgen receptor AR (but not ESR1) and synergizing with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity during skeletal muscle differentiation. It also serves as a coactivator for p53/TP53 in DNA damage response and apoptosis, and for RUNX2 in osteoblast differentiation. Additionally, DDX5 exhibits promoter-specific transcriptional repression likely through HDAC1 interaction, and as part of a PER complex, inhibits 3' transcriptional termination of circadian genes like PER1 and NR1D1 to modulate circadian rhythms.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human DDX5 at 2.5 μg/mL (100μL/well) on the plate can bind Anti- DDX5 Antibody. The ED50 for this effect is 20-100 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human DDX5 at 2.5 μg/mL (100 μL/well) on the plate can bind Anti-DDX5 Antibody. The ED50 for this effect is 36.69 ng/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-10*His;C-Myc
  • Accession

    P17844-1 (M1-Q614)

  • Gene ID
    1655
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    DDX5; DEAD (Asp-Glu-Ala-Asp) Box Helicase 5; Prev. G17P1; Putative ATP-Dependent RNA Helicase DDX5; Prev. HLR1; ATP-Dependent RNA Helicase DDX5; Probable ATP-Dependent RNA Helicase DDX5; RNA Helicase; DEAD Box Protein 5; DEAD Box-5; P68; HUMP68; DEAD/H (A

  • AA Sequence

    MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ

  • Molecular Weight

    Approximately 74-75 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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=
Mass (in vial) Mass (in vial)
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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human DDX5 at 2.5 μg/mL (100 μL/well) on the plate can bind Anti-DDX5 Antibody. The ED50 for this effect is 36.69 ng/mL.

  • DDX5 Protein, Human (His, Myc) Product Image
    1/16
  • Experimental Validation Results for DDX5 Protein, Human (His, Myc)
  • Experimental Validation Results for DDX5 Protein, Human (His, Myc)
  • DDX5 Protein, Human (His, Myc) Product Image