DENND1A Protein, Human (His-SUMO)

The DENND1A protein is a guanine nucleotide exchange factor (GEF) that coordinates clathrin-mediated endocytosis through activation of RAB35. It catalyzes the conversion of inactive GDP-bound RAB35 into its active GTP-bound state, regulating synaptic vesicle dynamics and other processes. DENND1A Protein, Human (His-SUMO) is the recombinant human-derived DENND1A, expressed by E. coli, with N-SUMO, N-6*His labeled tag. The total length of DENND1A Protein, Human (His-SUMO) is 559 a.a..

DENND1A, a guanine nucleotide exchange factor (GEF), plays a pivotal role in orchestrating clathrin-mediated endocytosis through the activation of RAB35. Its function involves catalyzing the exchange of GDP to GTP, thereby converting the inactive GDP-bound form of RAB35 into its active GTP-bound state. Notably, DENND1A is a key regulator of clathrin-mediated endocytosis processes, influencing synaptic vesicle dynamics and facilitating the exit from early endosomes. Additionally, DENND1A exhibits binding affinity for phosphatidylinositol-phosphates (PtdInsPs), with a preference for PtdIns(3)P. Intriguingly, its GEF activity is subject to autoinhibition, possibly mediated by an intramolecular interaction between the DENN domain and the C-terminus, with activation being regulated by Akt activation through disruption of this autoinhibitory mechanism.

Human

E. coli

N-SUMO;N-6*His

Q8TEH3-2 (M1-H559)

57706  [NCBI]

Full Length of Isoform-2

MGSRIKQNPETTFEVYVEVAYPRTGGTLSDPEVQRQFPEDYSDQEVLQTLTKFCFPFYVDSLTVSQVGQNFTFVLTDIDSKQRFGFCRLSSGAKSCFCILSYLPWFEVFYKLLNILADYTTKRQENQWNELLETLHKLPIPDPGVSVHLSVHSYFTVPDTRELPSIPENRNLTEYFVAVDVNNMLHLYASMLYERRILIICSKLSTLTACIHGSAAMLYPMYWQHVYIPVLPPHLLDYCCAPMPYLIGIHLSLMEKVRNMALDDVVILNVDTNTLETPFDDLQSLPNDVISSLKNRLKKVSTTTGDGVARAFLKAQAAFFGSYRNALKIEPEEPITFCEEAFVSHYRSGAMRQFLQNATQLQLFKQFIDGRLDLLNSGEGFSDVFEEEINMGEYAGSDKLYHQWLSTVRKGSGAILNTVKTKANPAMKTVYKFAKDHAKMGIKEVKNRLKQKDIAENGCAPTPEEQLPKTAPSPLVEAKDPKLREDRRPITVHFGQVRPPRPHVVKRPKSNIAVEGRRTSVPSPEQNTIATPATLHILQKSITHFAAKFPTRGWTSSSH

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.