DENND1A Protein, Human (His-SUMO)
The DENND1A protein is a guanine nucleotide exchange factor (GEF) that coordinates clathrin-mediated endocytosis through activation of RAB35. It catalyzes the conversion of inactive GDP-bound RAB35 into its active GTP-bound state, regulating synaptic vesicle dynamics and other processes. DENND1A Protein, Human (His-SUMO) is the recombinant human-derived DENND1A, expressed by E. coli, with N-SUMO, N-6*His labeled tag. The total length of DENND1A Protein, Human (His-SUMO) is 559 a.a..
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The DENND1A protein is a guanine nucleotide exchange factor (GEF) that coordinates clathrin-mediated endocytosis through activation of RAB35. It catalyzes the conversion of inactive GDP-bound RAB35 into its active GTP-bound state, regulating synaptic vesicle dynamics and other processes. DENND1A Protein, Human (His-SUMO) is the recombinant human-derived DENND1A, expressed by E. coli, with N-SUMO, N-6*His labeled tag. The total length of DENND1A Protein, Human (His-SUMO) is 559 a.a..
Background
DENND1A, a guanine nucleotide exchange factor (GEF), plays a pivotal role in orchestrating clathrin-mediated endocytosis through the activation of RAB35. Its function involves catalyzing the exchange of GDP to GTP, thereby converting the inactive GDP-bound form of RAB35 into its active GTP-bound state. Notably, DENND1A is a key regulator of clathrin-mediated endocytosis processes, influencing synaptic vesicle dynamics and facilitating the exit from early endosomes. Additionally, DENND1A exhibits binding affinity for phosphatidylinositol-phosphates (PtdInsPs), with a preference for PtdIns(3)P. Intriguingly, its GEF activity is subject to autoinhibition, possibly mediated by an intramolecular interaction between the DENN domain and the C-terminus, with activation being regulated by Akt activation through disruption of this autoinhibitory mechanism.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-SUMO;N-6*His
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Accession
Q8TEH3-2 (M1-H559)
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Molecular Construction
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N-term
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6*His-SUMO
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DENND1A (1M-559H)
Accession # Q8TEH3 -
C-term
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Protein Length
Full Length of Isoform-2
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Synonyms
DENND1A; DENN Domain-Containing Protein 1A; Prev. KIAA1608; FLJ21129; FAM31A; Protein FAM31A; Connecdenn 1; Connecdenn; DENN Domain Containing 1A; DENN/MADD Domain Containing 1A
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AA Sequence
MGSRIKQNPETTFEVYVEVAYPRTGGTLSDPEVQRQFPEDYSDQEVLQTLTKFCFPFYVDSLTVSQVGQNFTFVLTDIDSKQRFGFCRLSSGAKSCFCILSYLPWFEVFYKLLNILADYTTKRQENQWNELLETLHKLPIPDPGVSVHLSVHSYFTVPDTRELPSIPENRNLTEYFVAVDVNNMLHLYASMLYERRILIICSKLSTLTACIHGSAAMLYPMYWQHVYIPVLPPHLLDYCCAPMPYLIGIHLSLMEKVRNMALDDVVILNVDTNTLETPFDDLQSLPNDVISSLKNRLKKVSTTTGDGVARAFLKAQAAFFGSYRNALKIEPEEPITFCEEAFVSHYRSGAMRQFLQNATQLQLFKQFIDGRLDLLNSGEGFSDVFEEEINMGEYAGSDKLYHQWLSTVRKGSGAILNTVKTKANPAMKTVYKFAKDHAKMGIKEVKNRLKQKDIAENGCAPTPEEQLPKTAPSPLVEAKDPKLREDRRPITVHFGQVRPPRPHVVKRPKSNIAVEGRRTSVPSPEQNTIATPATLHILQKSITHFAAKFPTRGWTSSSH
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Predicted Molecular Mass
79.4 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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