ECE-2 Protein, Human (HEK293, His)

The ECE-2 protein plays a crucial role in vascular regulation by catalyzing the conversion of large endothelin-1 to endothelin-1, helping to regulate blood pressure and vascular tone. Furthermore, ECE-2 exhibits potential methyltransferase activity and may be involved in amyloid-β processing, suggesting that this enzyme has a broader impact on cellular function than its established role in endothelin processing. ECE-2 Protein, Human (HEK293, His) is the recombinant human-derived ECE-2 protein, expressed by HEK293 , with N-His labeled tag. The total length of ECE-2 Protein, Human (HEK293, His) is 685 a.a., with molecular weight of 120-125 kDa.

The ECE-2 protein is an essential enzyme with dual functionality. Primarily, it plays a crucial role in converting big endothelin-1 to endothelin-1, a biologically active peptide involved in the regulation of vascular tone and blood pressure. In addition to its role in the endothelin system, ECE-2 exhibits potential methyltransferase activity, implying its involvement in the transfer of methyl groups between molecules. Moreover, there is a suggested role for ECE-2 in amyloid-beta processing, indicating a possible association with processes related to neurodegenerative conditions. The multifaceted nature of ECE-2's activities underscores its significance in both cardiovascular physiology, through endothelin processing, and potential contributions to molecular events associated with neurodegenerative disorders (

Measured by its ability to cleave a peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read). The specific activity is 6005.388 pmol/min/µg, as measured under the described conditions.

Human

HEK293

N-10*His

P0DPD8/NP_055508.3 (G199-W883)

110599583  [NCBI]

GVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Approximately 120-125 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.