EEF1A1 Protein, Human (His-SUMO)

EEF1A1 is a translation elongation factor that catalyzes GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the ribosomal A site during protein synthesis. This involves mRNA codon-aa-tRNA anticodon base pairing, leading to GTP hydrolysis and aa-tRNA release in EEF1A1. EEF1A1 Protein, Human (His-SUMO) is the recombinant human-derived EEF1A1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

EEF1A1, a translation elongation factor, plays a crucial role in protein synthesis by catalyzing the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase. This process involves base pairing between the mRNA codon and the aa-tRNA anticodon, leading to GTP hydrolysis and the subsequent release of aa-tRNA from EEF1A1. The freed aa-tRNA is then accommodated into the ribosome, facilitating the extension of the growing protein chain through ribosome-catalyzed peptide bond formation. Beyond its core translational function, EEF1A1 participates in the positive regulation of IFNG transcription in T-helper 1 cells, forming an IFNG promoter-binding complex with TXK and PARP1. Moreover, in the context of microbial infection, EEF1A1 is essential for the translation of viral proteins and the replication of human coronavirus SARS-CoV-2, highlighting its critical role in both cellular and pathogenic translational processes.

Human

E. coli

N-6*His;N-SUMO

P68104 (M1-K462)

1915  [NCBI]

Full Length

MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK

Approximately 66-74 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.