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EEF1A1 Protein, Human (His-SUMO)

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EEF1A1 is a translation elongation factor that catalyzes GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the ribosomal A site during protein synthesis. This involves mRNA codon-aa-tRNA anticodon base pairing, leading to GTP hydrolysis and aa-tRNA release in EEF1A1. EEF1A1 Protein, Human (His-SUMO) is the recombinant human-derived EEF1A1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for EEF1A1 Protein, Human (His-SUMO)
  • EEF1A1 Protein, Human (His-SUMO) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

EEF1A1 is a translation elongation factor that catalyzes GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the ribosomal A site during protein synthesis. This involves mRNA codon-aa-tRNA anticodon base pairing, leading to GTP hydrolysis and aa-tRNA release in EEF1A1. EEF1A1 Protein, Human (His-SUMO) is the recombinant human-derived EEF1A1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

Background

EEF1A1, a translation elongation factor, plays a crucial role in protein synthesis by catalyzing the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase. This process involves base pairing between the mRNA codon and the aa-tRNA anticodon, leading to GTP hydrolysis and the subsequent release of aa-tRNA from EEF1A1. The freed aa-tRNA is then accommodated into the ribosome, facilitating the extension of the growing protein chain through ribosome-catalyzed peptide bond formation. Beyond its core translational function, EEF1A1 participates in the positive regulation of IFNG transcription in T-helper 1 cells, forming an IFNG promoter-binding complex with TXK and PARP1. Moreover, in the context of microbial infection, EEF1A1 is essential for the translation of viral proteins and the replication of human coronavirus SARS-CoV-2, highlighting its critical role in both cellular and pathogenic translational processes.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His;N-SUMO
  • Accession

    P68104 (M1-K462)

  • Gene ID
    1915  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-SUMO
    • EEF1A1 (M1-K462)
      Accession # P68104
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    EEF1A1; Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14; Prev. EEF1A; Translation Elongation Factor 1 Alpha 1-Like 14; Prev. LENG7; Epididymis Secretory Sperm Binding Protein; Prev. EF1A; Elongation Factor 1 Alpha Subunit; Elongation Factor 1-A

  • AA Sequence

    MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK

  • Molecular Weight

    Approximately 66-74 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • EEF1A1 Protein, Human (His-SUMO) Product Image
    1/16
  • Experimental Validation Results for EEF1A1 Protein, Human (His-SUMO)
  • EEF1A1 Protein, Human (His-SUMO) Product Image