EIF4A2 Protein, Human (GST)
EIF4A2, an ATP-dependent RNA helicase, is a vital subunit of the eIF4F complex, pivotal in cap recognition and mRNA binding to ribosomes. In translation initiation, EIF4A2 crucially unwinds RNA secondary structures in mRNA 5'-UTRs. This activity facilitates small ribosomal subunit binding and efficient scanning for the initiator codon, orchestrating translation initiation. EIF4A2 Protein, Human (GST) is the recombinant human-derived EIF4A2 protein, expressed by E. coli , with N-GST labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
EIF4A2, an ATP-dependent RNA helicase, is a vital subunit of the eIF4F complex, pivotal in cap recognition and mRNA binding to ribosomes. In translation initiation, EIF4A2 crucially unwinds RNA secondary structures in mRNA 5'-UTRs. This activity facilitates small ribosomal subunit binding and efficient scanning for the initiator codon, orchestrating translation initiation. EIF4A2 Protein, Human (GST) is the recombinant human-derived EIF4A2 protein, expressed by E. coli , with N-GST labeled tag.
EIF4A2, an ATP-dependent RNA helicase, operates as a crucial subunit within the eIF4F complex, playing a pivotal role in cap recognition and facilitating the binding of mRNA to ribosomes. In the established model of translation initiation, EIF4A2 takes on the responsibility of unwinding RNA secondary structures present in the 5'-UTR of mRNAs. This unwinding activity is essential to create a conducive environment for the efficient binding of the small ribosomal subunit and subsequent scanning for the initiator codon, thus orchestrating the intricate process of translation initiation.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-GST
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Accession
Q14240 (M1-I407)
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Molecular Construction
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N-term
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GST
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EIF4A2 (M1-I407)
Accession # Q14240 -
C-term
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Protein Length
Full Length
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Synonyms
EIF4A2; ATP-Dependent RNA Helicase EIF4A-2; Prev. EIF4F; EIF4A-II; DDX2B; Eukaryotic Translation Initiation Factor 4A, Isoform 2; BM-010; ATP-Dependent RNA Helicase; EIF4A; RNA Helicase; Eukaryotic Initiation Factor 4A-II; NEDHSS; Eukaryotic Translation I
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AA Sequence
MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI
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Predicted Molecular Mass
73.4 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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