ENPP-1 Protein, Mouse (HEK293, His)
Based on 1 publication(s) in Google Scholar
ENPP-1 is a nucleotide pyrophosphatase that critically regulates bone mineralization and soft tissue calcification through the production of diphosphate (PPi).This PPi inhibits the growth of hydroxyapatite crystals and prevents unwanted mineralization.ENPP-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-1 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ENPP-1 is a nucleotide pyrophosphatase that critically regulates bone mineralization and soft tissue calcification through the production of diphosphate (PPi).This PPi inhibits the growth of hydroxyapatite crystals and prevents unwanted mineralization.ENPP-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-1 protein, expressed by HEK293 , with C-His labeled tag.
Background
The ENPP-1 protein, a nucleotide pyrophosphatase, plays a crucial role in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. It generates diphosphate (PPi), which inhibits the mineralization of bone and calcification of soft tissues by binding to nascent hydroxyapatite crystals, preventing their further growth. ENPP-1 exhibits preferential hydrolysis of ATP but can also hydrolyze other nucleoside 5' triphosphates and diadenosine polyphosphates. Additionally, it participates in the regulation of nucleotide sugars in the endoplasmic reticulum and Golgi, impacting purinergic signaling. The protein inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling, although the direct or indirect nature of hedgehog inhibition remains unclear. ENPP-1 is implicated in modulating insulin sensitivity and melanogenesis. Notably, it can hydrolyze 2',3'-cGAMP, a second messenger activating TMEM173/STING and triggering type-I interferon production, though the precise role of this hydrolysis in the extracellular space is not fully understood. However, ENPP-1 is unable to hydrolyze the 2',3'-cGAMP linkage isomer 3',3'-cGAMP.
Verified Bioactivity
Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is >100000 pmol/min/μg, as measured under the described conditions.
Publications (1)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-8*His
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Accession
P06802-1 (K80-D906)
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Molecular Construction
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N-term
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ENPP-1 (K80-D906)
Accession # P06802-1 -
8*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ENPP1; Nucleotide Diphosphatase; Prev. PDNP1; NPPase; Prev. M6S1; Membrane Component, Chromosome 6, Surface Marker 1; Prev. NPPS; Membrane Component Chromosome 6 Surface Marker 1; PC-1; Plasma-Cell Membrane Glycoprotein 1; PCA1; Alkaline Phosphodiesterase
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AA Sequence
KPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDVDDIYHMTVPYGRPRILLKQHHVCLLQQQQFLTGYSLDLLMPLWASYTFLRNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED
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Predicted Molecular Mass
96 kDa
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Molecular Weight
Approximately 100-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (241 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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