Enpp1 - ectonucleotide pyrophosphatase/phosphodiesterase 1 Gene

Also Known as Pca; ttw; twy; M6S1; NPP1; Npps; PC-1; Ly-41; Pca-1; Pdnp1; CD203c; E-NPP1; E-NPP 1; 4833416E15Rik

Species: Mus musculus

Gene Type: protein coding

Gene ID: 18605

Summary

This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of Enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II Transmembrane Glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the Insulin Receptor, inhibits downstream signaling events and induces Insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Enpp1 Products (3)

mRNA	Protein	Name
NM_001308327.1	NP_001295256.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform 1
NM_001308329.1	NP_001295258.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform 3
NM_008813.4	NP_032839.3	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform 2

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables calcium ion binding	IDA IDA: Inferred from direct assay	23027977	MGI
enables cyclic-GMP-AMP hydrolase activity	IDA IDA: Inferred from direct assay	30356045	MGI
enables cyclic-GMP-AMP hydrolase activity	IMP IMP: Inferred from mutant phenotype	25344812	MGI
enables dinucleotide phosphatase activity	IDA IDA: Inferred from direct assay	23027977	MGI
enables identical protein binding	IPI IPI: Inferred from physical interaction	23041369	MGI
enables nucleoside triphosphate diphosphatase activity	IDA IDA: Inferred from direct assay	30356045	MGI
enables phosphatase activity	IDA IDA: Inferred from direct assay	12533192	MGI
enables phosphatase activity	IMP IMP: Inferred from mutant phenotype	23798568	MGI
enables phosphodiesterase I activity	IDA IDA: Inferred from direct assay	1647027	MGI
enables phosphodiesterase I activity	IMP IMP: Inferred from mutant phenotype	24770645	MGI
enables phosphoric diester hydrolase activity	IDA IDA: Inferred from direct assay	12533192	MGI
enables protein homodimerization activity	IDA IDA: Inferred from direct assay	12533192	MGI
enables pyrophosphatase activity	IDA IDA: Inferred from direct assay	7678057	MGI
enables zinc ion binding	IDA IDA: Inferred from direct assay	23027977	MGI

Biological Process GO Annotation	Evidence	References	Source
involved in ATP metabolic process	IDA IDA: Inferred from direct assay	23027977	MGI
involved in B-1 B cell homeostasis	IMP IMP: Inferred from mutant phenotype	23169635	MGI
involved in D-glucose import	IMP IMP: Inferred from mutant phenotype	29259245	MGI
involved in T cell differentiation	IMP IMP: Inferred from mutant phenotype	23169635	MGI
involved in Wnt signaling pathway	IMP IMP: Inferred from mutant phenotype	32980560	MGI
involved in adipose tissue development	IMP IMP: Inferred from mutant phenotype	25368121	MGI
involved in adult locomotory behavior	IMP IMP: Inferred from mutant phenotype	26624227	MGI
involved in adult walking behavior	IMP IMP: Inferred from mutant phenotype	22927960	MGI
involved in aorta development	IMP IMP: Inferred from mutant phenotype	22359666	MGI
involved in apoptotic process involved in development	IMP IMP: Inferred from mutant phenotype	21935678	MGI
involved in artery development	IMP IMP: Inferred from mutant phenotype	24530784	MGI
involved in articular cartilage development	IMP IMP: Inferred from mutant phenotype	22359666	MGI
involved in axon regeneration	IMP IMP: Inferred from mutant phenotype	21935678	MGI
involved in biomineral tissue development	IMP IMP: Inferred from mutant phenotype	22359666	MGI
involved in bone development	IMP IMP: Inferred from mutant phenotype	28798354	MGI
involved in bone growth	IMP IMP: Inferred from mutant phenotype	9541520	MGI
acts upstream of bone mineralization	IDA IDA: Inferred from direct assay	21930712	MGI
involved in bone mineralization	IDA IDA: Inferred from direct assay	12817751	MGI
involved in bone mineralization	IGI IGI: Inferred from genetic interaction	12082181	MGI
acts upstream of bone mineralization	IMP IMP: Inferred from mutant phenotype	21930712	MGI
involved in bone mineralization	IMP IMP: Inferred from mutant phenotype	12082181	MGI
involved in bone mineralization involved in bone maturation	IMP IMP: Inferred from mutant phenotype	22359666	MGI
acts upstream of or within bone remodeling	IMP IMP: Inferred from mutant phenotype	10024383	MGI
involved in bone remodeling	IMP IMP: Inferred from mutant phenotype	9541520	MGI
involved in bone resorption	IMP IMP: Inferred from mutant phenotype	28798354	MGI
involved in bone trabecula formation	IMP IMP: Inferred from mutant phenotype	22359666	MGI
involved in calcium ion homeostasis	IGI IGI: Inferred from genetic interaction	22927960	MGI
involved in calcium ion homeostasis	IMP IMP: Inferred from mutant phenotype	12817751	MGI
involved in cartilage development	IMP IMP: Inferred from mutant phenotype	15625282	MGI
acts upstream of positive effect cell morphogenesis	IDA IDA: Inferred from direct assay	21930712	MGI
acts upstream of positive effect cell morphogenesis	IMP IMP: Inferred from mutant phenotype	21930712	MGI
involved in cell population proliferation	IGI IGI: Inferred from genetic interaction	22927960	MGI
involved in cell population proliferation	IMP IMP: Inferred from mutant phenotype	22927960	MGI
involved in cellular homeostasis	IGI IGI: Inferred from genetic interaction	12082181	MGI
involved in cellular homeostasis	IMP IMP: Inferred from mutant phenotype	12082181	MGI
involved in cellular response to sodium phosphate	IMP IMP: Inferred from mutant phenotype	28798354	MGI
involved in cementum mineralization	IDA IDA: Inferred from direct assay	31059863	MGI
involved in cementum mineralization	IGI IGI: Inferred from genetic interaction	31059863	MGI
involved in cementum mineralization	IMP IMP: Inferred from mutant phenotype	31059863	MGI
involved in central nervous system myelination	IMP IMP: Inferred from mutant phenotype	21935678	MGI
involved in collagen-activated signaling pathway	IMP IMP: Inferred from mutant phenotype	32980560	MGI
involved in defense response to protozoan	IMP IMP: Inferred from mutant phenotype	29259245	MGI
involved in determination of adult lifespan	IMP IMP: Inferred from mutant phenotype	23798568	MGI
involved in diphosphate metabolic process	IDA IDA: Inferred from direct assay	31059863	MGI
involved in diphosphate metabolic process	IMP IMP: Inferred from mutant phenotype	31059863	MGI
involved in endochondral bone morphogenesis	IMP IMP: Inferred from mutant phenotype	22359666	MGI
involved in endochondral ossification	IMP IMP: Inferred from mutant phenotype	24770645	MGI
involved in establishment of localization in cell	IMP IMP: Inferred from mutant phenotype	27959908	MGI
involved in fat cell differentiation	IMP IMP: Inferred from mutant phenotype	24770645	MGI
involved in fat pad development	IMP IMP: Inferred from mutant phenotype	24770645	MGI
involved in fatty acid metabolic process	IMP IMP: Inferred from mutant phenotype	31730234	MGI
involved in fibroblast growth factor receptor signaling pathway	IMP IMP: Inferred from mutant phenotype	32980560	MGI
acts upstream of positive effect gene expression	IDA IDA: Inferred from direct assay	21930712	MGI
involved in gene expression	IDA IDA: Inferred from direct assay	23169635	MGI
involved in gene expression	IGI IGI: Inferred from genetic interaction	24530784	MGI
involved in gene expression	IMP IMP: Inferred from mutant phenotype	12817751	MGI
involved in glucose homeostasis	IMP IMP: Inferred from mutant phenotype	25368121	MGI
involved in glycolytic process	IMP IMP: Inferred from mutant phenotype	29259245	MGI
involved in heart development	IMP IMP: Inferred from mutant phenotype	26624227	MGI
involved in hematopoietic stem cell migration to bone marrow	IMP IMP: Inferred from mutant phenotype	31666661	MGI
involved in hormone metabolic process	IMP IMP: Inferred from mutant phenotype	25368121	MGI
involved in immunoglobulin production involved in immunoglobulin-mediated immune response	IMP IMP: Inferred from mutant phenotype	23169635	MGI
involved in inflammatory response to antigenic stimulus	IMP IMP: Inferred from mutant phenotype	29259245	MGI
involved in inhibition of non-skeletal tissue mineralization	IDA IDA: Inferred from direct assay	21490328	MGI
involved in inhibition of non-skeletal tissue mineralization	IGI IGI: Inferred from genetic interaction	24732453	MGI
involved in inhibition of non-skeletal tissue mineralization	IMP IMP: Inferred from mutant phenotype	15625282	MGI
involved in inorganic diphosphate transport	IDA IDA: Inferred from direct assay	28701330	MGI
involved in inorganic diphosphate transport	IGI IGI: Inferred from genetic interaction	28704395	MGI
involved in inorganic diphosphate transport	IMP IMP: Inferred from mutant phenotype	28701330	MGI
involved in kidney development	IMP IMP: Inferred from mutant phenotype	22359666	MGI
involved in leukocyte activation involved in inflammatory response	IMP IMP: Inferred from mutant phenotype	29259245	MGI
involved in ligamentous ossification	IMP IMP: Inferred from mutant phenotype	21935678	MGI
involved in long-term synaptic potentiation	IMP IMP: Inferred from mutant phenotype	31730234	MGI
involved in mRNA transcription by RNA polymerase II	IMP IMP: Inferred from mutant phenotype	22359666	MGI
involved in macrophage differentiation	IMP IMP: Inferred from mutant phenotype	23717624	MGI
involved in magnesium ion homeostasis	IMP IMP: Inferred from mutant phenotype	28402956	MGI
involved in metabolic process	IMP IMP: Inferred from mutant phenotype	28798354	MGI
involved in microglia differentiation	IMP IMP: Inferred from mutant phenotype	23717624	MGI
involved in microglial cell migration	IMP IMP: Inferred from mutant phenotype	31666661	MGI
involved in middle ear morphogenesis	IMP IMP: Inferred from mutant phenotype	27959908	MGI
involved in mitochondrion organization	IMP IMP: Inferred from mutant phenotype	25368121	MGI
involved in morphogenesis of an epithelium	IMP IMP: Inferred from mutant phenotype	27959908	MGI
involved in mucus secretion	IMP IMP: Inferred from mutant phenotype	27959908	MGI
involved in multicellular organism growth	IMP IMP: Inferred from mutant phenotype	9541520	MGI
involved in muscle cell cellular homeostasis	IMP IMP: Inferred from mutant phenotype	15625282	MGI
involved in negative regulation of bone mineralization	IMP IMP: Inferred from mutant phenotype	22510396	MGI
involved in negative regulation of fat cell differentiation	IMP IMP: Inferred from mutant phenotype	17849011	MGI
involved in negative regulation of hh target transcription factor activity	IMP IMP: Inferred from mutant phenotype	30111653	MGI
acts upstream of or within negative regulation of ossification	IMP IMP: Inferred from mutant phenotype	9662402	MGI
involved in neuron apoptotic process	IMP IMP: Inferred from mutant phenotype	21935678	MGI
involved in odontogenesis	IMP IMP: Inferred from mutant phenotype	29533727	MGI
involved in oligodendrocyte apoptotic process	IMP IMP: Inferred from mutant phenotype	21935678	MGI
involved in organic phosphonate metabolic process	IMP IMP: Inferred from mutant phenotype	26763447	MGI
acts upstream of ossification	IMP IMP: Inferred from mutant phenotype	9662402	MGI
involved in ossification	IMP IMP: Inferred from mutant phenotype	27959908	MGI
involved in osteoblast differentiation	IDA IDA: Inferred from direct assay	21930712	MGI
involved in osteoblast differentiation	IMP IMP: Inferred from mutant phenotype	15920156	MGI
involved in osteoclast differentiation	IMP IMP: Inferred from mutant phenotype	29533727	MGI
involved in phosphate ion homeostasis	IDA IDA: Inferred from direct assay	28701330	MGI
involved in phosphate ion homeostasis	IGI IGI: Inferred from genetic interaction	12082181	MGI
involved in phosphate ion homeostasis	IMP IMP: Inferred from mutant phenotype	12082181	MGI
involved in plasma cell differentiation	IMP IMP: Inferred from mutant phenotype	29259245	MGI
involved in post-embryonic forelimb morphogenesis	IMP IMP: Inferred from mutant phenotype	24770645	MGI
acts upstream of or within negative effect protein poly-ADP-ribosylation	IDA IDA: Inferred from direct assay	27406238	MGI
involved in response to Gram-positive bacterium	IMP IMP: Inferred from mutant phenotype	23169635	MGI
involved in response to dietary excess	IMP IMP: Inferred from mutant phenotype	25368121	MGI
involved in response to insulin	IMP IMP: Inferred from mutant phenotype	25368121	MGI
involved in response to magnesium ion	IGI IGI: Inferred from genetic interaction	24732453	MGI
involved in response to magnesium ion	IMP IMP: Inferred from mutant phenotype	23798568	MGI
involved in response to platelet-derived growth factor	IMP IMP: Inferred from mutant phenotype	24530784	MGI
involved in response to sodium phosphate	IGI IGI: Inferred from genetic interaction	12082181	MGI
involved in response to sodium phosphate	IMP IMP: Inferred from mutant phenotype	12082181	MGI
involved in response to vitamin B6	IGI IGI: Inferred from genetic interaction	12082181	MGI
involved in response to wounding	IMP IMP: Inferred from mutant phenotype	24530784	MGI
involved in sensory perception of mechanical stimulus	IMP IMP: Inferred from mutant phenotype	31666661	MGI
involved in sensory perception of sound	IMP IMP: Inferred from mutant phenotype	27959908	MGI
involved in sensory perception of temperature stimulus	IMP IMP: Inferred from mutant phenotype	31666661	MGI
involved in skin development	IMP IMP: Inferred from mutant phenotype	28798354	MGI
involved in smoothened signaling pathway	IMP IMP: Inferred from mutant phenotype	32980560	MGI
involved in spinal cord development	IMP IMP: Inferred from mutant phenotype	21935678	MGI
involved in tooth mineralization	IMP IMP: Inferred from mutant phenotype	29533727	MGI
involved in type II interferon production	IMP IMP: Inferred from mutant phenotype	23169635	MGI
involved in vascular associated smooth muscle cell migration	IMP IMP: Inferred from mutant phenotype	24530784	MGI
involved in vascular associated smooth muscle cell proliferation	IMP IMP: Inferred from mutant phenotype	24530784	MGI
involved in vasculogenesis	IMP IMP: Inferred from mutant phenotype	25260930	MGI
involved in vitamin D3 metabolic process	IMP IMP: Inferred from mutant phenotype	28798354	MGI

Cellular Component GO Annotation	Evidence	References	Source
is active in cell surface	IDA IDA: Inferred from direct assay	23169635	MGI
located in cell surface	IDA IDA: Inferred from direct assay	8223581	MGI
is active in extracellular region	IMP IMP: Inferred from mutant phenotype	12817751	MGI
located in extracellular space	IDA IDA: Inferred from direct assay	8223581	MGI
located in membrane	IDA IDA: Inferred from direct assay	1647027	MGI
is active in plasma membrane	IDA IDA: Inferred from direct assay	12533192	MGI
located in plasma membrane	IDA IDA: Inferred from direct assay	3104326	MGI

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

Protein Preferred Names	Protein Names
ectonucleotide pyrophosphatase/phosphodiesterase family member 1	lymphocyte antigen 41 phosphodiesterase I/nucleotide pyrophosphatase 1 plasma-cell membrane glycoprotein PC-1 tiptoe walking

Recombinant Enpp1 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P700709	ENPP-1 Protein, Mouse (HEK293, His)	P06802-1 (K80-D906)	≥ 95%, as determined by Bis-Tris PAGE.

Related Agents

Clinical Phase
Pre-clinical Phase

Clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Phase
HY-176708	ISM5939	Inhibitor	99.79%	Phase 1

Inhibitory Antibodies (1)

Cat. No.	Product Name	Effect	Purity	Phase
HY-P991677	Efmirenpase alfa		/	Phase 3

Pre-clinical Phase

Bioactive Molecules (42)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-129490	Enpp-1-IN-1	Inhibitor	99.19%	Unkown
HY-147389	Enpp-1-IN-14	Inhibitor	99.67%	Unkown
HY-139362	Enpp-1-IN-2	Inhibitor	99.51%	Unkown
HY-143256	Enpp-1-IN-12	Inhibitor	99.28%	Unkown
HY-141831	STF-1084	Inhibitor	99.92%	Unkown
HY-155457	Enpp-1-IN-19		99.28%	Unkown
HY-169804	ENPP3 Inhibitor 1	Inhibitor	99.43%	Unkown
HY-158791	Enpp-1-IN-21	Inhibitor	99.07%	Unkown
HY-153335	Enpp-1-IN-16	Inhibitor	98.46%	Unkown
HY-163343	Enpp-1-IN-20		98.06%	Unkown
HY-176296	Vizenpistat	Inhibitor	99.14%	Unkown
HY-143254	Enpp-1-IN-10	Inhibitor	98.64%	Unkown
HY-149465	STF-1623	Inhibitor	99.90%	Unkown
HY-145063	Enpp-1-IN-5	Inhibitor	98.16%	Unkown
HY-153398	Enpp-1-IN-17	Inhibitor	99.65%	Unkown
HY-173189	2-5A	Inhibitor	/	Unkown
HY-143255	Enpp-1-IN-11	Inhibitor	/	Unkown
HY-145062	Enpp-1-IN-4	Inhibitor	/	Unkown
HY-145064	Enpp-1-IN-6	Inhibitor	/	Unkown
HY-145065	Enpp-1-IN-7	Inhibitor	/	Unkown
HY-145069	Enpp-1-IN-8	Inhibitor	/	Unkown
HY-145070	Enpp-1-IN-9	Inhibitor	/	Unkown
HY-147998	Enpp-1-IN-13	Inhibitor	/	Unkown
HY-153334	Enpp-1-IN-15	Inhibitor	/	Unkown
HY-157522	NCI-14465	Inhibitor	/	Unkown
HY-162911	Enpp-1-IN-22	Inhibitor	/	Unkown
HY-162912	Enpp-1-IN-23	Inhibitor	/	Unkown
HY-168147	Enpp-1-IN-24	Inhibitor	/	Unkown
HY-168491	Enpp-1-IN-25		/	Unkown
HY-172527	CD73-IN-20	Inhibitor	/	Unkown
HY-172528	Enpp-1/3-IN-1	Inhibitor	/	Unkown
HY-175047	Enpp-1-IN-26	Inhibitor	/	Unkown
HY-175478	Enpp-1-IN-27		/	Unkown
HY-176828	Enpp-1-IN-28	Inhibitor	/	Unkown
HY-176874	Enpp-1-IN-29	Inhibitor	/	Unkown
HY-181667	Enpp-1-IN-30	Inhibitor	/	Unkown
HY-182772	Enpp-1-IN-31	Inhibitor	/	Unkown
HY-184200	Enpp-1-IN-32		/	Unkown
HY-RS04387	ENPP1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-156205	CdnP-IN-1	Inhibitor	/	Unkown
HY-RS16545	Enpp1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS22977	Enpp1 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Homo sapiens	Enpp1	NCBI	NCBI:5167