Enpp-1-IN-28
Enpp-1-IN-28 is a ENPP1 inhibitor at the molecular and cellular levels, with IC50 values of 0.188 µM and 0.732 µM, respectively. Enpp-1-IN-28 upgrades the expression levels of IFN-β in vivo by preventing the ENPP1 from hydrolyzing the cGAMP to stimulate a more potent innate immune response. Enpp-1-IN-28 can be used for the study of metastatic breast cancer.
For research use only. We do not sell to patients.
- Formula: C17H13FN4O2S
- Molecular Weight:356.37
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
ENPP1 0.188 μM (IC50, molecular levels) |
ENPP1 0.732 μM (IC50, cellular levels) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| MDA-MB-231 | IC50 |
<1 μM
Compound: 53
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Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by CCK8 assay
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[PMID: 38359537] |
In Vitro
Enpp-1-IN-28 (Compound 4e) displays cytotoxic activity against 4T1 cell line (IC50 = 2.99 μM), while exerting nonsignificant cytotoxic effect on normal cell lines (LO2 and 293T) at 50 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:4T1 breast cancer cells were subcutaneously implanted into 6-8-week-old BALB/c nude mice[1]
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Dosage:0.5 mg/kg combined with 5 mg/kg cGAMP
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Administration:i.v. for a single dose
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Result:Increased the expression level of IFN-β in the serum of BALB/c nude mice compared with the free cGAMP group.
Protected cGAMP from degradation by ENPP1, thereby stimulating a more potent innate immune response.
Chemical Information
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Molecular Weight 356.37
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Formula C17H13FN4O2S
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SMILES
FC1=CC2=C(C(NC(CSC3=NC4=CC(OC)=CC=C4N3)=N2)=O)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Enpp-1-IN-28 Related Classifications
HY-176828 Related Classifications
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