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EPCR Protein, Human (HEK293, hFc)

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Based on 1 publication(s) in Google Scholar

EPCR Protein binds activated protein C, enhancing its activation by the thrombin-thrombomodulin complex, and is pivotal in the protein C pathway, regulating blood coagulation. EPCR Protein, Human (HEK293, hFc) is the recombinant human-derived EPCR protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for EPCR Protein, Human (HEK293, hFc)
  • EPCR Protein, Human (HEK293, hFc) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

EPCR Protein binds activated protein C, enhancing its activation by the thrombin-thrombomodulin complex, and is pivotal in the protein C pathway, regulating blood coagulation. EPCR Protein, Human (HEK293, hFc) is the recombinant human-derived EPCR protein, expressed by HEK293 , with C-hFc labeled tag.

Background

EPCR protein is capable of binding activated protein C and acts to enhance its activation by the thrombin-thrombomodulin complex. This protein plays a crucial role in the protein C pathway, which regulates blood coagulation.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human EPCR at 2 μg/mL (100 μL/well) can bind Anti-EPCR antibody. The ED50 for this effect is 18.21 ng/mL.

MCE Validation Data

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human EPCR at 2 μg/mL (100 μL/well) can bind Anti-EPCR antibody. The ED50 for this effect is 18.21 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-hFc
  • Accession

    Q9UNN8 (S18-T209)

  • Gene ID
    10544  [NCBI]
  • Molecular Construction
    • N-term
    • EPCR (S18-T209)
      Accession # Q9UNN8
    • hFc
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    PROCR; Protein C Receptor, Endothelial; Protein C Receptor; CD201 Antigen; EPCR; APC Receptor; Endothelial Protein C Receptor; CD201; Activated Protein C Receptor; Cell Cycle, Centrosome-Associated Protein; CCD41; Centrocyclin; Endothelial Cell Protein C

  • AA Sequence

    SQDASDGLQRLHMLQISYFRDPYHVWYQGNASLGGHLTHVLEGPDTNTTIIQLQPLQEPESWARTQSGLQSYLLQFHGLVRLVHQERTLAFPLTIRCFLGCELPPEGSRAHVFFEVAVNGSSFVSFRPERALWQADTQVTSGVVTFTLQQLNAYNRTRYELREFLEDTCVQYVQKHISAENTKGSQTSRSYT

  • Predicted Molecular Mass

    48.9 kDa

  • Molecular Weight

    Approximately 120 kDa & 65 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human EPCR at 2 μg/mL (100 μL/well) can bind Anti-EPCR antibody. The ED50 for this effect is 18.21 ng/mL.

  • EPCR Protein, Human (HEK293, hFc) Product Image
    1/16
  • Experimental Validation Results for EPCR Protein, Human (HEK293, hFc)
  • EPCR Protein, Human (HEK293, hFc) Product Image