EphB1 Protein, Mouse (HEK293, His)

EphB1 protein is a receptor tyrosine kinase that participates in bidirectional signaling with ephrin B ligands (including EFNB1, EFNB2, and EFNB3). It plays a crucial role in retinal axon guidance, neural progenitor cell regulation, dendritic spine maturation, synapse formation, angiogenesis, targeted cell migration, and muscle stem cell maintenance. EphB1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphB1 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphB1 Protein, Mouse (HEK293, His) is 522 a.a., with molecular weight of ~65-75 kDa.

EphB1 protein is a receptor tyrosine kinase that binds to ephrin-B ligands on neighboring cells, resulting in contact-dependent bidirectional signaling. This receptor mediates both forward signaling and reverse signaling pathways. Its ligands include EFNB1, EFNB2, and EFNB3, which play important roles in various cellular processes. During nervous system development, EphB1 regulates retinal axon guidance and interacts with EFNB2. In the adult nervous system, it works together with EFNB3 to regulate chemotaxis, proliferation, and polarity of neural progenitors. EphB1 is also involved in dendritic spine maturation, synapse formation, angiogenesis, targeted cell migration, and adhesion. Activation by EFNB1 and other ephrin-B ligands activates MAPK/ERK and JNK signaling pathways, which regulate cell migration and adhesion. Additionally, EphB1 is important for maintaining the pool of muscle stem cells (satellite cells) by promoting self-renewal and reducing activation and differentiation.

Immobilized mouse EPHB1 10 μg/mL (100 μL/well) can bind mouse EFNB1, The ED₅₀ for this effect is 128.3 ng/mL.

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  Immobilized mouse EPHB1 10 μg/mL (100 μL/well) can bind mouse EFNB1, The ED₅₀ for this effect is 128.3 ng/mL.

Mouse

HEK293

C-His

Q8CBF3 (M18-L539)

270190  [NCBI]

MEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQL

Approximately 65-75 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.