EphB1 Protein, Mouse (HEK293, His)
Based on 1 publication(s) in Google Scholar
EphB1 protein is a receptor tyrosine kinase that participates in bidirectional signaling with ephrin B ligands (including EFNB1, EFNB2, and EFNB3). It plays a crucial role in retinal axon guidance, neural progenitor cell regulation, dendritic spine maturation, synapse formation, angiogenesis, targeted cell migration, and muscle stem cell maintenance. EphB1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphB1 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
EphB1 protein is a receptor tyrosine kinase that participates in bidirectional signaling with ephrin B ligands (including EFNB1, EFNB2, and EFNB3). It plays a crucial role in retinal axon guidance, neural progenitor cell regulation, dendritic spine maturation, synapse formation, angiogenesis, targeted cell migration, and muscle stem cell maintenance. EphB1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphB1 protein, expressed by HEK293 , with C-His labeled tag.
EphB1 protein is a receptor tyrosine kinase that binds to ephrin-B ligands on neighboring cells, resulting in contact-dependent bidirectional signaling. This receptor mediates both forward signaling and reverse signaling pathways. Its ligands include EFNB1, EFNB2, and EFNB3, which play important roles in various cellular processes. During nervous system development, EphB1 regulates retinal axon guidance and interacts with EFNB2. In the adult nervous system, it works together with EFNB3 to regulate chemotaxis, proliferation, and polarity of neural progenitors. EphB1 is also involved in dendritic spine maturation, synapse formation, angiogenesis, targeted cell migration, and adhesion. Activation by EFNB1 and other ephrin-B ligands activates MAPK/ERK and JNK signaling pathways, which regulate cell migration and adhesion. Additionally, EphB1 is important for maintaining the pool of muscle stem cells (satellite cells) by promoting self-renewal and reducing activation and differentiation.
1.This product does not contain protein kinase domain.
2.Immobilized mouse EPHB1 10 μg/mL (100 μL/well) can bind mouse EFNB1, The ED50 for this effect is 128.3 ng/mL.
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Cancer Commun (Lond)
Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma. [Abstract]2025 Feb;45(2):143-166. PMID: 39648610
Technical Parameters
-
Species Mouse
-
Source HEK293
-
Tag C-His
-
Accession
Q8CBF3-1 (M18-L539)
-
Molecular Construction
-
N-term
-
EphB1 (M18-L539)
Accession # Q8CBF3-1 -
His
-
C-term
-
-
Protein Length
Extracellular Domain
-
Synonyms
EPHB1; ELK; Prev. EPHT2; EK6; Hek6; Receptor Protein-Tyrosine Kinase; Neuronally-Expressed EPH-Related Tyrosine Kinase; Eph Tyrosine Kinase 2; Tyrosine-Protein Kinase Receptor EPH-2; EPH Tyrosine Kinase 2; Ephrin Type-B Receptor 1; EphB1; EPH-Like Kinase
-
AA Sequence
MEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQL
-
Molecular Weight
Approximately 64 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (239 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
