EphB3 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The EphB3 protein is a receptor tyrosine kinase that performs bidirectional signaling with the transmembrane ephrin B ligand. It participates in forward signaling and reverse signaling, sharing functionality with EPHB2. EphB3 Protein, Human (HEK293, His) is the recombinant human-derived EphB3 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The EphB3 protein is a receptor tyrosine kinase that performs bidirectional signaling with the transmembrane ephrin B ligand. It participates in forward signaling and reverse signaling, sharing functionality with EPHB2. EphB3 Protein, Human (HEK293, His) is the recombinant human-derived EphB3 protein, expressed by HEK293 , with C-His labeled tag.
Background
The EphB3 protein is a receptor tyrosine kinase that engages in contact-dependent bidirectional signaling with adjacent cells by binding to transmembrane ephrin-B family ligands. This receptor is involved in both forward signaling, downstream of the receptor, and reverse signaling, downstream of the ephrin ligand. EphB3 shares overlapping and redundant functions with EPHB2, particularly in axon guidance during development, where it regulates the formation of interhemispheric connections in the cerebral cortex. Additionally, EphB3 contributes to the development and maturation of dendritic spines and excitatory synapses, as well as controls cell migration and positioning in various developmental processes such as angiogenesis, palate development, and thymic epithelium development. The EFNB2/EPHB3 complex also plays a role in regulating migration and adhesion of cells involved in the tubularization of the urethra and septation of the cloaca. Lastly, EphB3 is crucial for intestinal epithelium differentiation, distinguishing progenitor cells from differentiated cells within the crypt.
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Immobilized Human EphB3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-8*His
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Accession
P54753 (G34-L559)
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Molecular Construction
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N-term
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EphB3 (G34-L559)
Accession # P54753 -
8*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
EPHB3; Embryonic Kinase 2; Prev. ETK2; Human Embryo Kinase 2; Tyro6; EPH-Like Kinase 2; Hek2; EphB3; Tyrosine-Protein Kinase TYRO6; TYRO6; EPH-Like Tyrosine Kinase 2; HEK2; Ephrin Type-B Receptor 3; EPH Receptor B3; EK2
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AA Sequence
GCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIPNAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLRLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSEGIASTVTSQMNSVQLDGLRPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPL
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Predicted Molecular Mass
58.6 kDa
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Molecular Weight
Approximately 65-68 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5, 8% trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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