EphB6 Protein, Mouse (HEK293, His)

EphB6 Protein, a kinase-defective receptor, binds ephrin-B1 and ephrin-B2, influencing cell adhesion and migration with nuanced effects. Its interaction with ephrin-B2 inhibits JNK activation, T-cell receptor-induced IL-2 secretion, and CD25 expression. EphB6 also interacts with CBL, EPHB1, and ligand-independently with FYN, highlighting its intricate role in cellular signaling pathways. EphB6 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphB6 protein, expressed by HEK293 , with C-His labeled tag.

EphB6, a kinase-defective receptor, serves as a binding partner for members of the ephrin-B family, specifically interacting with ephrin-B1 and ephrin-B2. Its engagement with ephrin-B2 leads to a nuanced modulation of cell adhesion and migration, eliciting both positive and negative effects. EphB6 plays a role in inhibiting JNK activation, T-cell receptor-induced IL-2 secretion, and CD25 expression upon stimulation with ephrin-B2. Furthermore, EphB6 engages in interactions with CBL and EPHB1, and notably associates with FYN in a ligand-independent manner, emphasizing its intricate involvement in cellular signaling pathways.

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized Mouse EphB6 at 10 μg/mL (100 μL/well) can bind Mouse EFNB1. The ED₅₀ for this effect is 0.3034 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Mouse EphB6 at 10 μg/mL (100 μL/well) can bind Mouse EFNB1. The ED₅₀ for this effect is 0.3034 μg/mL.

Mouse

HEK293

C-His

O08644 (L33-S587)

13848  [NCBI]

Extracellular Domain

LEEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTRTFEACHVAGLPPGSGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQADEPDGPDSIAAWHLKRWTKVDTIAADESFPASSSSSSWAVGPHRTGQRVGLQLNVKERSFGPLTQRGFYVAFQDTGACLALVAVKLFSYTCPSVLRAFASFPETQASGAGGASLVAAVGTCVAHAEPEEDGVGGQAGGSPPRLHCNGEGRWMVAVGGCRCQPGHQPARGDKLCQACPEGSYKALAGNVPCSPCPARSHSPDPAAPVCPCLQGFYRASSDPPEAPCTGPPSAPRELWFEVQGSALMLHWRLPQELGGRGDLLFNVVCKECGGHGEPSSGGMCRRCRDEVHFDPRQRGLTESRVLVGGLRAHVPYILEVQAVNGVSELSPDPPQAAAINVSTSHEVPSAVPVMHQVSRAANSITVSWPQPEQTNGNILDYQLRYYDQAEDESHSFTMTSETNTATVTRLSPGHIYGFQVRARTAAGHGPYGGKVYFQTLPQGELSSQLPEKLS

Approximately 66 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.