Fetuin B Protein, Human (HEK293, His)

Fetuin B, a vital protease inhibitor, plays a crucial role in egg fertilization by preventing premature zona pellucida hardening before fertilization. Its primary function involves inhibiting the protease activity of ASTL, preventing ZP2 cleavage and zona pellucida hardening. Fetuin B's regulatory role emphasizes its significance in creating optimal conditions for successful fertilization and subsequent embryonic development. Fetuin B Protein, Human (HEK293, His) is the recombinant human-derived Fetuin B protein, expressed by HEK293 , with C-6*His labeled tag.

Fetuin B emerges as a crucial protease inhibitor essential for egg fertilization. Its primary role is to prevent premature hardening of the zona pellucida before fertilization, a critical process for successful embryonic development. This function is likely mediated through the inhibition of the protease activity of ASTL, a key enzyme responsible for cleaving ZP2 and triggering the hardening of the zona pellucida. Fetuin B's regulatory role in this process highlights its significance in facilitating the proper conditions for successful fertilization and subsequent embryonic development.

Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-Leu-Arg-AMC. The IC₅₀ value is 21.16 nM, as measured under the described conditions.

Human

HEK293

C-6*His

Q9UGM5-1 (C16-P382)

26998  [NCBI]

Full Length of Isoform-1 Mature Protein

CGAMSPPQLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYNNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPP

Approximately 53-55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.