Fibulin-3 Protein, Mouse (HEK293, His)

Fibulin-3 protein plays multiple roles, binding to EGFR to induce downstream signaling and affecting cell adhesion and migration. It may negatively regulate chondrocyte differentiation and affect glial cell function in the olfactory epithelium, including migration, differentiation, and support of neurite outgrowth. Fibulin-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Fibulin-3 protein, expressed by HEK293 , with N-His labeled tag. The total length of Fibulin-3 Protein, Mouse (HEK293, His) is 476 a.a., with molecular weight of 60-80 kDa.

The Fibulin-3 Protein exhibits a multifaceted role by binding to EGFR, the EGF receptor, leading to EGFR autophosphorylation and the subsequent activation of downstream signaling pathways. This interaction suggests its involvement in crucial cellular processes such as cell adhesion and migration. Additionally, Fibulin-3 may function as a negative regulator of chondrocyte differentiation and, in the olfactory epithelium, potentially regulates glial cell migration, differentiation, and the ability of glial cells to support neuronal neurite outgrowth. Furthermore, it interacts with ECM1 and TIMP3, indicating its participation in intricate molecular networks that contribute to various cellular functions.

Mouse

HEK293

N-His

Q8BPB5 (Q18-F493)

216616  [NCBI]

QYTEETITYTQCTDGYEWDPIRQQCKDIDECDIVPDACKGGMKCVNHYGGYLCLPKTAQIIVNNEHPQQETPAAEASSGATTGTVAARSMATSGVVPGGGFMASATAVAGPEVQTGRNNFVIRRNPADPQRIPSNPSHRIQCAAGYEQSEHNVCQDIDECTSGTHNCRTDQVCINLRGSFTCQCLPGYQKRGEQCVDIDECTVPPYCHQRCVNTPGSFYCQCSPGFQLAANNYTCVDINECDASNQCAQQCYNILGSFICQCNQGYELSSDRLNCEDIDECRTSSYLCQYQCVNEPGKFSCMCPQGYEVVRSRTCQDINECETTNECREDEMCWNYHGGFRCYPRNPCQDHYVLTSENRCVCPVSNTMCRELPQSIVYKYMSIRSDRSVPSDIFQIQATMIYANTINTFRIKSGNENGEFYLRQTSPVSAMLVLVKSLSGPREYIVDLEMLTVSSIGTFRTSSVLRLTIIVGPFSF

60-80 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.