FKBP12 Protein, Human (His)
Based on 1 Customer Validation
FKBP12 protein maintains TGFBR1 in an inactive state, inhibits activin signaling by recruiting SMAD7, and modulates RYR1 calcium channel activity. As a PPIase, it speeds up protein folding. FKBP12's multifunctionality underscores its regulatory role in cellular processes, emphasizing its importance in signaling and protein folding. FKBP12, Human (His) is the recombinant human-derived FKBP12 protein, expressed by E. coli, with C-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FKBP12 protein maintains TGFBR1 in an inactive state, inhibits activin signaling by recruiting SMAD7, and modulates RYR1 calcium channel activity. As a PPIase, it speeds up protein folding. FKBP12's multifunctionality underscores its regulatory role in cellular processes, emphasizing its importance in signaling and protein folding. FKBP12, Human (His) is the recombinant human-derived FKBP12 protein, expressed by E. coli, with C-His labeled tag.
Background
FKBP12 protein plays a crucial role in maintaining the inactive conformation of TGFBR1, the serine/threonine kinase receptor for TGF-beta, effectively preventing receptor activation in the absence of ligand. Additionally, FKBP12 recruits SMAD7 to ACVR1B, inhibiting the association of SMAD2 and SMAD3 with the activin receptor complex and thereby blocking activin signaling. This multifunctional protein may also modulate the activity of the RYR1 calcium channel. As a peptidyl-prolyl cis-trans isomerase (PPIase), FKBP12 accelerates the folding of proteins by catalyzing the isomerization of proline imidic peptide bonds in oligopeptides. The diverse functions of FKBP12 highlight its regulatory role in various cellular processes, emphasizing its significance in intracellular signaling and protein folding.
Verified Bioactivity
Measured by its ability to convert the substrate, Suc-AAPF-pNA (HY-P2573), from Cis to Trans formation. The specific activity is ≥2000 pmol/min/μg.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P62942/NP_463460.1 (M1-E108)
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Gene ID2280
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Molecular Construction
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N-term
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FKBP12 (M1-E108)
Accession # P62942/NP_463460.1 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
FKBP1A; Calstabin-1; Prev. FKBP1; FKBP12C; FKBP-12; FKBP-1A; FKBP12; FK506-Binding Protein, T-Cell, 12-KD; PPIASE; FK506-Binding Protein 1A (12kD); PKC12; Protein Kinase C Inhibitor 2; Peptidyl-Prolyl Cis-Trans Isomerase FKBP1A; FK506 Binding Protein 1A;
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AA Sequence
MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE
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Predicted Molecular Mass
12.8 kDa
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Molecular Weight
Approximately 13 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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