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  4. FLG Protein, Human (P.pastoris, His)

FLG Protein, Human (P.pastoris, His)

Cat. No.: HY-P71734
Handling Instructions

FLG Protein, essential in the mammalian epidermis, plays a key role in terminal differentiation. It aggregates keratin intermediate filaments and promotes disulfide bond formation, emphasizing its crucial contribution to the structural organization and integrity of the epidermis in the concluding stages of cellular differentiation. FLG Protein, Human (P.pastoris, His) is the recombinant human-derived FLG protein, expressed by P. pastoris , with N-His labeled tag. The total length of FLG Protein, Human (P.pastoris, His) is 224 a.a., with molecular weight of ~26.8 kDa.

For research use only. We do not sell to patients.

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Description

FLG Protein, essential in the mammalian epidermis, plays a key role in terminal differentiation. It aggregates keratin intermediate filaments and promotes disulfide bond formation, emphasizing its crucial contribution to the structural organization and integrity of the epidermis in the concluding stages of cellular differentiation. FLG Protein, Human (P.pastoris, His) is the recombinant human-derived FLG protein, expressed by P. pastoris , with N-His labeled tag. The total length of FLG Protein, Human (P.pastoris, His) is 224 a.a., with molecular weight of ~26.8 kDa.
Background

FLG, a crucial protein in the mammalian epidermis, plays a pivotal role in the terminal differentiation process by aggregating keratin intermediate filaments and facilitating the formation of disulfide bonds among these filaments. This function underscores the significance of FLG in the structural organization and integrity of the epidermis during the final stages of cellular differentiation.
Species

Human
Source

P. pastoris
Tag

N-His
Accession

P20930 (3838D-4061E)
Gene ID
Molecular Construction
N-term
His
FLG (3838D-4061E)
Accession # P20930
C-term
Synonyms
ATOD2; Epidermal filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin
AA Sequence

DSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE

Molecular Weight

Approximately 26.8 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

FLG Protein, Human (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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