FLT3 Protein, Cynomolgus (HEK293, His)
FLT3 is a tyrosine-protein kinase receptor that regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. It phosphorylates downstream effectors such as SHC1 and AKT1, activating signaling cascades involving MTOR, RAS, and MAP kinases. FLT3 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived FLT3 protein, expressed by HEK293, with C-His labeled tag.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FLT3 is a tyrosine-protein kinase receptor that regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. It phosphorylates downstream effectors such as SHC1 and AKT1, activating signaling cascades involving MTOR, RAS, and MAP kinases. FLT3 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived FLT3 protein, expressed by HEK293, with C-His labeled tag.
Background
FLT3, a tyrosine-protein kinase, functions as a cell-surface receptor for the cytokine FLT3LG, exerting regulatory control over the differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. This receptor facilitates the phosphorylation of various downstream effectors, including SHC1 and AKT1, and activates signaling cascades involving MTOR, RAS, MAPK1/ERK2, and/or MAPK3/ERK1. Moreover, it plays a pivotal role in the phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. While wild-type FLT3 activation leads to modest STAT5A or STAT5B activation, mutations causing constitutive kinase activity result in heightened cell proliferation and resistance to apoptosis, underscoring its role in fostering aberrant signaling pathways.
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-His
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Accession
A0A2K5VQ48 (Q28-S541)
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Gene ID/
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Molecular Construction
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N-term
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FLT3 (Q28-S541)
Accession # A0A2K5VQ48 -
His
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C-term
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Protein Length
Partial
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Synonyms
FLT3; CD135 Antigen; Fms Related Receptor Tyrosine Kinase 3; FLK-2; CD135; Growth Factor Receptor Tyrosine Kinase Type III; STK1; Receptor Protein-Tyrosine Kinase; FLK2; Fms-Related Tyrosine Kinase 3; Fms-Like Tyrosine Kinase 3; Fetal Liver Kinase 2; Receptor-Type Tyrosine-Protein Kinase FLT3; Fetal Liver Kinase-2; Fms Related Tyrosine Kinase 3; STK-1; Stem Cell Tyrosine Kinase 1; FLT-3; FL Cytokine Receptor
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AA Sequence
QDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSAGTVYEAATVEVDVSASITLQVLVDTPGNISCLWVFKHSSLNCQPHFDVQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTIVFTVSIRNTLLFTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGTDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGSYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAYPQIRCTWTFSRKAFPCEQKALDDGYSVSKFCNHKHQPGEYIFHAENGDAQFTKMFTLNIRRKPQVLAEASASQASCSSDGYPLPSWTWKKCSDKSPNCTEDIPEGVWNRKANRKVFGQWVSSSTLNMSEAMKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDS
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Predicted Molecular Mass
59.9 kDa
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Molecular Weight
Approximately 85 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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