FLT3 Protein, Human (Active, Biotinylated, sf9, His, Avi)
FLT3 is a tyrosine-protein kinase receptor that regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. It phosphorylates downstream effectors such as SHC1 and AKT1, activating signaling cascades involving MTOR, RAS, and MAP kinases. FLT3 Protein, Human (Biotinylated, sf9, His, Avi) is the recombinant human-derived FLT3, expressed by Sf9 insect cells, with Avi, His labeled tag. The total length of FLT3 Protein, Human (Biotinylated, sf9, His, Avi) is 370 a.a..
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FLT3 is a tyrosine-protein kinase receptor that regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. It phosphorylates downstream effectors such as SHC1 and AKT1, activating signaling cascades involving MTOR, RAS, and MAP kinases. FLT3 Protein, Human (Biotinylated, sf9, His, Avi) is the recombinant human-derived FLT3, expressed by Sf9 insect cells, with Avi, His labeled tag. The total length of FLT3 Protein, Human (Biotinylated, sf9, His, Avi) is 370 a.a..
Background
FLT3, a tyrosine-protein kinase, functions as a cell-surface receptor for the cytokine FLT3LG, exerting regulatory control over the differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. This receptor facilitates the phosphorylation of various downstream effectors, including SHC1 and AKT1, and activates signaling cascades involving MTOR, RAS, MAPK1/ERK2, and/or MAPK3/ERK1. Moreover, it plays a pivotal role in the phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. While wild-type FLT3 activation leads to modest STAT5A or STAT5B activation, mutations causing constitutive kinase activity result in heightened cell proliferation and resistance to apoptosis, underscoring its role in fostering aberrant signaling pathways.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-Avi;N-8*His
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Accession
P36888-1 (H564-S993)
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Gene ID2322
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Protein Length
Cytoplasmic Domain
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Conjugation
Biotin
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Synonyms
FLT3; CD135 Antigen; Fms Related Receptor Tyrosine Kinase 3; FLK-2; CD135; Growth Factor Receptor Tyrosine Kinase Type III; STK1; Receptor Protein-Tyrosine Kinase; FLK2; Fms-Related Tyrosine Kinase 3; Fms-Like Tyrosine Kinase 3; Fetal Liver Kinase 2; Receptor-Type Tyrosine-Protein Kinase FLT3; Fetal Liver Kinase-2; Fms Related Tyrosine Kinase 3; STK-1; Stem Cell Tyrosine Kinase 1; FLT-3; FL Cytokine Receptor
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AA Sequence
MPALARDGGQLPLLVVFSAMIFGTITNQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNTLLYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGTDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISKFCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRKPQVLAEASASQASCFSDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNMSEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDNISFYATIGVCLLFIVVLTLLICHKYKKQFRYESQLQMVQVTGSSDNEYFYVDFREYEYDLKWEFPRENLEFGKVLGSGAFGKVMNATAYGISKTGVSIQVAVKMLKEKADSSEREALMSELKMMTQLGSHENIVNLLGACTLSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHPNSSMPGSREVQIHPDSDQISGLHGNSFHSEDEIEYENQKRLEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVTHGKVVKICDFGLARDIMSDSNYVVRGNARLPVKWMAPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGIPVDANFYKLIQNGFKMDQPFYATEEIYIIMQSCWAFDSRKRPSFPNLTSFLGCQLADAEEAMYQNVDGRVSECPHTYQNRRPFSREMDLGLLSPQAQVEDS
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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