1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CX3CL1
  5. Fractalkine/CX3CL1 Protein, Rat

Fractalkine/CX3CL1 Protein, Rat

Cat. No.: HY-P79193
COA Handling Instructions

Fractalkine/CX3CL1 protein is a versatile chemokine that acts as a ligand for CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. It regulates immune response, inflammation, adhesion, and chemotaxis. It activates integrins via CX3CR1-dependent and -independent pathways, binding to site 1 with CX3CR1 and site 2 without CX3CR1. Soluble Fractalkine/CX3CL1 attracts T-cells and monocytes, not neutrophils. Fractalkine/CX3CL1 Protein, Rat is the recombinant rat-derived Fractalkine/CX3CL1 protein, expressed by E. coli , with tag free. The total length of Fractalkine/CX3CL1 Protein, Rat is 79 a.a., with molecular weight of ~11 kDa.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Fractalkine/CX3CL1 protein is a versatile chemokine that acts as a ligand for CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. It regulates immune response, inflammation, adhesion, and chemotaxis. It activates integrins via CX3CR1-dependent and -independent pathways, binding to site 1 with CX3CR1 and site 2 without CX3CR1. Soluble Fractalkine/CX3CL1 attracts T-cells and monocytes, not neutrophils. Fractalkine/CX3CL1 Protein, Rat is the recombinant rat-derived Fractalkine/CX3CL1 protein, expressed by E. coli , with tag free. The total length of Fractalkine/CX3CL1 Protein, Rat is 79 a.a., with molecular weight of ~11 kDa.

Background

Fractalkine/CX3CL1 protein serves as a multifaceted chemokine, acting as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling cascade exhibits diverse functions across various tissue compartments, encompassing roles in immune response modulation, inflammation regulation, cell adhesion, and chemotaxis. Specifically, it plays a crucial role in regulating leukocyte adhesion and migration processes at the endothelium. Fractalkine/CX3CL1 demonstrates the ability to activate integrins in a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, it activates integrins by binding to the classical ligand-binding site (site 1) in integrins. Conversely, in the absence of CX3CR1, it binds to a distinct site (site 2) on integrins, enhancing the binding of other integrin ligands to site 1. The soluble form of Fractalkine/CX3CL1 exhibits chemotactic properties for T-cells and monocytes, though not for neutrophils.

Biological Activity

Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 5.736 ng/mL, corresponding to a specific activity is 1.74×10^5 U/mg.

  • Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells.The ED50 this effect is 5.736 ng/mL,corresponding to a specific activity is 1.74×105 U/mg.
Species

Rat

Source

E. coli

Tag

Tag Free

Accession

O55145 (L22-G100)

Gene ID
Molecular Construction
N-term
Fractalkine (L22-G100)
Accession # O55145
C-term
Synonyms
Fractalkine; Cx3cl1; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1; Acc1; Fkn; Scyd1
AA Sequence

LAGQHLGMTKCNITCHKMTSPIPVTLLIHYQLNQESCGKRAIILETRQHRHFCADPKEKWVQDAMKHLDHQTAALTRNG

Molecular Weight

Approximately 11 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.8.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Fractalkine/CX3CL1 Protein, Rat Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Fractalkine/CX3CL1 Protein, Rat
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HY-P79193
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