G-CSFR/CD114 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The G-CSFR/CD114 protein, as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocyte maturation and plays a key role in the proliferation, differentiation, and survival of neutrophil lineage cells. Furthermore, it may be involved in cell surface adhesion or recognition events. G-CSFR/CD114 Protein, Human (HEK293, His) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The G-CSFR/CD114 protein, as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocyte maturation and plays a key role in the proliferation, differentiation, and survival of neutrophil lineage cells. Furthermore, it may be involved in cell surface adhesion or recognition events. G-CSFR/CD114 Protein, Human (HEK293, His) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293 , with C-His labeled tag.
Background
G-CSFR/CD114, the receptor for granulocyte colony-stimulating factor (CSF3), holds a pivotal role in orchestrating granulocytic maturation by governing the proliferation, differentiation, and survival of cells along the neutrophilic lineage. This homodimeric receptor engages with two CSF3 molecules, forming a crucial interaction that regulates essential cellular processes. Beyond its involvement in cellular maturation, G-CSFR/CD114 may contribute to adhesion or recognition events at the cell surface. Furthermore, its interaction with CEACAM1 serves as a regulatory axis, down-regulating the CSF3R-STAT3 pathway by recruiting PTPN6, which subsequently dephosphorylates CSF3R and modulates cellular signaling dynamics.
Verified Bioactivity
Immobilized Human G-CSF R, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of ≤ 6.0 ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q99062-1 (E25-H627)
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Molecular Construction
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N-term
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CSF3R (E25-H627)
Accession # Q99062-1 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CSF3R; GCSFR; Prev. CD114; G-CSF Receptor; Granulocyte Colony-Stimulating Factor Receptor; CD114 Antigen; G-CSF-R; SCN7; Colony Stimulating Factor 3 Receptor; Colony Stimulating Factor 3 Receptor (Granulocyte)
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AA Sequence
ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTPEGSELH
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Predicted Molecular Mass
68.3 kDa
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Molecular Weight
Approximately 90-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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