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Galectin-8/LGALS8 Protein, Rat (His)

Customer Review

Based on 1 Customer Validation

Galectin-8 belongs to the family of animal lectins that bind to different glycoconjugates. Galectin-8 inhibits MTOR and AMPK signaling pathways to induce autophagy in response to lysosomal damage. Galectin-8 is highly expressed in a variety of tumors. Galectin-8 Protein, Rat (His) is the recombinant rat-derived Galectin-8, expressed by E. coli, with N-6*His labeled tag. The total length of Galectin-8 Protein, Rat (His) is 316 a.a..

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Galectin-8/LGALS8 Protein, Rat (His)
  • Experimental Validation Results for Galectin-8/LGALS8 Protein, Rat (His)
  • Galectin-8/LGALS8 Protein, Rat (His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Galectin-8 belongs to the family of animal lectins that bind to different glycoconjugates. Galectin-8 inhibits MTOR and AMPK signaling pathways to induce autophagy in response to lysosomal damage. Galectin-8 is highly expressed in a variety of tumors. Galectin-8 Protein, Rat (His) is the recombinant rat-derived Galectin-8, expressed by E. coli, with N-6*His labeled tag. The total length of Galectin-8 Protein, Rat (His) is 316 a.a..

Background

Galectin-8 belongs to the family of animal lectins that bind to different glycoconjugates. As a secreted protein, Galectin-8 is found in body fluids or the serum of patients with breast cancer and those with colon cancer. Extracellular Galectin-8 promotes cell adhesion by binding to cell adhesion molecules such as integrins CD44, CD166 and Podoplanin. Galectin-8 performs its intracellular function by marking the vacuoles invaded by pathogens to be destroyed by autophagy. Galectin-8 inhibited MTOR and AMPK in response to lysosomal injury induced autophagy in membrane disturbances. Galectin-8 is highly expressed in a variety of tumors[1][2][3].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized SLC31A1 at 5 μg/mL (100 μL/well) can bind Rat Galectin-8. The ED50 for this effect is 3.773 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized SLC31A1 at 5 μg/mL (100 μL/well) can bind Rat Galectin-8, The ED50 for this effect is 3.773 μg/mL.

Technical Parameters

  • Species Rat
  • Source E. coli
  • Tag N-6*His
  • Accession

    Q6IN24 (M1-W316)

  • Gene ID
    116641
  • Protein Length

    Full Length

  • Synonyms

    LGALS8; Galectin-8; Galectin 8; Po66-CBP; PCTA-1; Gal-8; Lectin, Galactoside-Binding, Soluble, 8; Po66 Carbohydrate Binding Protein; Prostate Carcinoma Tumor Antigen 1; Alternative Protein LGALS8; Po66 Carbohydrate-Binding Protein; Galectin; Galectin-8g;

  • AA Sequence

    MLSLSNLQNIIYNPTIPYVSTITEQLKPGSLIVIRGHVPKDSERFQVDFQHGNSLKPRADVAFHFNPRFKRSNCIVCNTLTNEKWGWEEITHDMPFRKEKSFEIVIMVLKNKFQVAVNGKHILLYAHRINPEKIDTLGIYGKVNIHSIGFRFSSDLQSMETSTLGLTQISKENIQKSGKLHLSLPFEARLNASMGPGRTVVVKGEVNTNAKSFNVDLVAGRSRDIALHLNPRLNVKAFVRNSFLQDAWGEEERNITCFPFSSGMYFEMIIYCDVREFKVAVNGVHSLEYKHRFKDLSSIDTLAVDGDIRLLDVRSW

  • Predicted Molecular Mass

    36.1 kDa

  • Molecular Weight

    Approximately 34 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 10% glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Zick Y. Galectin-8, cytokines, and the storm. Biochem Soc Trans. 2022 Feb 28;50(1):135-149. [Content Brief]

[2]. Storti P, et al. Role of Galectins in Multiple Myeloma. Int J Mol Sci. 2017 Dec 17;18(12):2740. [Content Brief]

[3]. Jia J, et al. Galectins control MTOR and AMPK in response to lysosomal damage to induce autophagy. Autophagy. 2019 Jan;15(1):169-171. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized SLC31A1 at 5 μg/mL (100 μL/well) can bind Rat Galectin-8, The ED50 for this effect is 3.773 μg/mL.

  • Galectin-8/LGALS8 Protein, Rat (His) Product Image
    1/16
  • Experimental Validation Results for Galectin-8/LGALS8 Protein, Rat (His)
  • Experimental Validation Results for Galectin-8/LGALS8 Protein, Rat (His)
  • Galectin-8/LGALS8 Protein, Rat (His) Product Image