GAP43 Protein, Human (HEK293, His)

The GAP43 protein plays a central role in nerve growth and is found primarily in the active "growth cones" at the tips of axons. It induces axonal and dendritic filopodia, which are critical for neurodevelopmental dynamics. GAP43 Protein, Human (HEK293, His) is the recombinant human-derived GAP43 protein, expressed by HEK293 , with C-His labeled tag. The total length of GAP43 Protein, Human (HEK293, His) is 238 a.a., with molecular weight of ~47 kDa.

GAP43 protein is intricately associated with nerve growth, emerging as a major component within the motile 'growth cones' that form at the tips of elongating axons. Its pivotal role extends to the induction of axonal and dendritic filopodia, contributing to the dynamic processes crucial for nerve development. Within a molecular context, GAP43 is identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, and CTTN, suggesting its participation in diverse signaling pathways. Additionally, GAP43 exhibits interactions with calmodulin, mediated by its IQ domain, binding calmodulin with greater affinity in the absence of Ca(2+) than in its presence. These intricate molecular associations underscore the multifaceted involvement of GAP43 in nerve growth and signaling cascades, shedding light on its significance in orchestrating axonal dynamics and filopodia formation.

Human

HEK293

C-His

P17677 (M1-A238)

2596  [NCBI]

Approximately 47 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.