GBP4 protein, Mouse (HEK293, His)
Based on 1 Customer Validation
GBP4, an IFN-inducible GTPase, crucially defends against various pathogens.It hydrolyzes GTP to GDP and aids inflammasome assembly by releasing ligands from bacteria.GBP4 promotes pathogen-containing vacuole lysis, facilitating cytosolic entry, where it recruits proteins like Gm12250/Irgb10 for bacterial cytolysis.This liberation activates inflammasomes, including CASP4/CASP11 and AIM2.GBP4 may also influence erythroid differentiation.GBP4 protein, mouse (HEK293, His) is the recombinant mouse-derived GBP4 protein, expressed by HEK293 , with N-8*His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
GBP4, an IFN-inducible GTPase, crucially defends against various pathogens.It hydrolyzes GTP to GDP and aids inflammasome assembly by releasing ligands from bacteria.GBP4 promotes pathogen-containing vacuole lysis, facilitating cytosolic entry, where it recruits proteins like Gm12250/Irgb10 for bacterial cytolysis.This liberation activates inflammasomes, including CASP4/CASP11 and AIM2.GBP4 may also influence erythroid differentiation.GBP4 protein, mouse (HEK293, His) is the recombinant mouse-derived GBP4 protein, expressed by HEK293 , with N-8*His labeled tag.
Background
GBP4 is an interferon (IFN)-inducible GTPase with crucial roles in innate immunity against a broad spectrum of bacterial, viral, and protozoan pathogens. It efficiently hydrolyzes GTP, predominantly yielding GDP. Upon infection, GBP4 is recruited to pathogen-containing vacuoles or vacuole-escaped bacteria, acting as a positive regulator of inflammasome assembly. This occurs by promoting the release of inflammasome ligands from bacteria. GBP4 facilitates pathogen-containing vacuole lysis, leading to the release of pathogens into the cytosol. In the cytosol, it promotes the recruitment of proteins, such as Gm12250/Irgb10, which mediate bacterial cytolysis. This liberation of ligands, including lipopolysaccharide (LPS) and double-stranded DNA (dsDNA), activates inflammasomes, such as the non-canonical CASP4/CASP11 inflammasome and AIM2 inflammasome, respectively. Additionally, GBP4 may play a role in erythroid differentiation.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag N-8*His
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Accession
Q61107 (E2-S620)
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Gene ID55932
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Molecular Construction
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N-term
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8*His
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GBP4 (E2-S620)
Accession # Q61107 -
C-term
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Protein Length
Full Length
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Synonyms
GBP3; GTP-Binding Protein 3; Guanylate Binding Protein 3; FLJ10961; Guanine Nucleotide-Binding Protein 3; Guanylate-Binding Protein 3 Delta C; Guanylate-Binding Protein 3; GBP-3
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AA Sequence
EAPICLVENWKNQLTVNLEAIRILEQIAQPLVVVAIVGLYRTGKSYLMNRLAGRNHGFSLGSTVQSETKGIWMWCVPHPTKPTHTLVLLDTEGLGDVEKGDPKNDSWIFALAVLLSSTFVYNSMSTINQQALEQLHFVTELTQLIRAKSSPREDKVKDSSEFVGFFPDFIWAVRDFALELKLNGRPITEDEYLENALKLIQGDNLKVQQSNMTRECIRYFFPVRKCFVFDRPTSDKRLLLQIENVPENQLERNFQVESEKFCSYIFTNGKTKTLRGGVIVTGNRLGTLVQTYVNAINSGTVPCLENAVTTLAQRENSIAVQKAADHYSEQMAQRMRLPTDTLQELLTVHAACEKEAIAVFMEHSFKDDEQEFQKKLVVTIEERKEEFIRQNEAASIRHCQAELERLSESLRKSISCGAFSVPGGHSLYLEARKKIELGYQQVLRKGVKAKEVLKSFLQSQAIMEDSILQSDKALTDGERAIAAERTKKEVAEKELELLRQRQKEQEQVMEAQERSFRENIAKLQEKMESEKEMLLREQEKMLEHKLKVQEELLIEGFREKSDMLKNEISHLREEMERTRRKPSLFGQILDTIGNAFIMILPGAGKLFGVGLKFLGSLSS
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Molecular Weight
Approximately 74 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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