GCDH Protein, Human (His)

The GCDH protein is essential for the degradation of L-lysine, L-hydroxylysine, and L-tryptophan. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, releasing CO(2). GCDH Protein, Human (His) is the recombinant human-derived GCDH protein, expressed by E. coli , with N-6*His labeled tag.

GCDH Protein plays a crucial role in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism by catalyzing the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, accompanied by the release of CO(2). This enzymatic process involves the utilization of electron transfer flavoprotein as its electron acceptor, highlighting the protein's dependence on this cofactor for efficient functioning within the metabolic pathway. It's noteworthy that the Short isoform of GCDH Protein is inactive, indicating the existence of multiple isoforms with varying functional properties in the regulation of this metabolic process.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q92947-1 (R45-K438)

2639  [NCBI]

Full Length of Isoform-1 Mature Protein

RPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK

Approximately 41.0 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.