GGT5 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The GGT5 protein is an important glycosaminoglycan lyase that selectively cleaves heparin and heparan sulfate using a β-elimination mechanism. It targets specific linkages, such as alpha-D-GlcNp2S6S(1->4) alpha-L-IdoAp2S in heparin and alpha-D-GlcNp2Ac(or 2S)6OH(1->4)beta- in heparan sulfate D-GlcAp. GGT5 Protein, Human (HEK293, His) is the recombinant human-derived GGT5 protein, expressed by HEK293 , with N-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The GGT5 protein is an important glycosaminoglycan lyase that selectively cleaves heparin and heparan sulfate using a β-elimination mechanism. It targets specific linkages, such as alpha-D-GlcNp2S6S(1->4) alpha-L-IdoAp2S in heparin and alpha-D-GlcNp2Ac(or 2S)6OH(1->4)beta- in heparan sulfate D-GlcAp. GGT5 Protein, Human (HEK293, His) is the recombinant human-derived GGT5 protein, expressed by HEK293 , with N-His labeled tag.
Background
Heparinase II is a glycosaminoglycan-cleaving protein that utilizes a beta-elimination mechanism to cleave both heparin and heparan sulfate. Specifically, it targets heparin at the alpha-D-GlcNp2S6S(1->4) alpha-L-IdoAp2S linkage and heparan sulfate at the alpha-D-GlcNp2Ac(or 2S)6OH(1->4)beta-D-GlcAp linkage. This enzymatic activity contributes to the regulation of glycosaminoglycan composition and function, impacting cellular processes influenced by these molecules. The precise cleavage sites underscore the specificity of Heparinase II in modifying the structure of heparin and heparan sulfate, implicating its role in diverse physiological and pathological contexts where these glycosaminoglycans are involved.
Verified Bioactivity
Measured by its ability to hydrolyze glutathione to Glu and Cys-Gly. The specific activity is 881.5814 pmol/min/µg, as measured under the described conditions.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag N-10*His
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Accession
P36269-1/NP_004112.2 (S30-Y586)
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Molecular Construction
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N-term
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His
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GGT5 (S30-Y586)
Accession # P36269/NP_004112.2 -
C-term
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Protein Length
Extracellular Domain
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Synonyms
GGT5; Gamma-Glutamyltranspeptidase 5; Prev. GGTLA1; GGT 5; Gamma-Glutamyltransferase-Like Activity 1; Gamma-Glutamyl Transpeptidase-Related Protein; GGT-REL; Gamma-Glutamyl Cleaving Enzyme; Gamma-Glutamyl Transpeptidase-Related Enzyme; GGT-Rel; Glutathion
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AA Sequence
SRHQAPCGPQAFAHAAVAADSKVCSDIGRAILQQQGSPVDATIAALVCTSVVNPQSMGLGGGVIFTIYNVTTGKVEVINARETVPASHAPSLLDQCAQALPLGTGAQWIGVPGELRGYAEAHRRHGRLPWAQLFQPTIALLRGGHVVAPVLSRFLHNSILRPSLQASTLRQLFFNGTEPLRPQDPLPWPALATTLETVATEGVEVFYTGRLGQMLVEDIAKEGSQLTLQDLAKFQPEVVDALEVPLGDYTLYSPPPPAGGAILSFILNVLRGFNFSTESMARPEGRVNVYHHLVETLKFAKGQRWRLGDPRSHPKLQNASRDLLGETLAQLIRQQIDGRGDHQLSHYSLAEAWGHGTGTSHVSVLGEDGSAVAATSTINTPFGAMVYSPRTGIILNNELLDLCERCPRGSGTTPSPVSGDRVGGAPGRCWPPVPGERSPSSMVPSILINKAQGSKLVIGGAGGELIISAVAQAIMSKLWLGFDLRAAIAAPILHVNSKGCVEYEPNFSQEVQRGLQDRGQNQTQRPFFLNVVQAVSQEGACVYAVSDLRKSGEAAGY
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Molecular Weight
Approximately 23-27 & 48-61.8 kDa due to the glycosylation
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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