GPT Protein, Rat (sf9, His)
GPT proteins play a key role in cellular nitrogen metabolism by catalyzing the reversible ammonia interaction between alanine and 2-oxoglutarate to produce pyruvate and glutamate products. This enzyme activity is an integral part of the dynamic balance of amino acid metabolism and contributes to the interconversion of key metabolites. GPT Protein, Rat (sf9, His) is the recombinant rat-derived GPT protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Species: Rat
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
GPT proteins play a key role in cellular nitrogen metabolism by catalyzing the reversible ammonia interaction between alanine and 2-oxoglutarate to produce pyruvate and glutamate products. This enzyme activity is an integral part of the dynamic balance of amino acid metabolism and contributes to the interconversion of key metabolites. GPT Protein, Rat (sf9, His) is the recombinant rat-derived GPT protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background
The GPT protein, also known as alanine transaminase or ALT, plays a crucial role in cellular nitrogen metabolism by catalyzing the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This enzymatic activity is central to the interconversion of amino acids and is particularly important for the regulation of nitrogen balance within the cell. GPT is actively involved in liver gluconeogenesis, contributing to the synthesis of glucose from precursors transported from skeletal muscles. The reversible transamination reactions mediated by GPT are integral to various metabolic pathways, emphasizing its significance in amino acid metabolism and glucose homeostasis. Understanding the functions of GPT provides insights into the dynamic regulation of nitrogen and carbon flux in cellular metabolism.
Technical Parameters
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Species Rat
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Source Sf9 insect cells
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Tag C-His
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Accession
P25409 (M1-S496)
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Molecular Construction
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N-term
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GPT (M1-S496)
Accession # P25409 -
His
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C-term
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Protein Length
Full Length
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Synonyms
GPT; Glutamate Pyruvate Transaminase 1; Glutamic--Pyruvic Transaminase; Glutamic--Pyruvic Transaminase 1; ALT1; Alanine Aminotransferase 1; GPT1; Alanine Aminotransferase; SGPT; Alanine Transaminase; ALT; AAT1; Glutamic-Pyruvate Transaminase (Alanine Amin
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AA Sequence
MASRVNDQSQASRNGLKGKVLTLDTMNPCVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFFRQVLALCVYPNLLSSPDFPEDAKRRAERILQACGGHSLGAYSISSGIQPIREDVAQYIERRDGGIPADPNNIFLSTGASDAIVTMLKLLVSGEGRARTGVLIPIPQYPLYSAALAELDAVQVDYYLDEERAWALDIAELRRALCQARDRCCPRVLCVINPGNPTGQVQTRECIEAVIRFAFKEGLFLMADEVYQDNVYAEGSQFHSFKKVLMEMGPPYSTQQELASFHSVSKGYMGECGFRGGYVEVVNMDAEVQKQMGKLMSVRLCPPVPGQALMDMVVSPPTPSEPSFKQFQAERQEVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPQVQLPLKAVQRAQELGLAPDMFFCLCLLEETGICVVPGSGFGQQEGTYHFRMTILPPMEKLRLLLEKLSHFHAKFTHEYS
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Predicted Molecular Mass
55 kDa
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Molecular Weight
Approximately 48 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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