Grancalcin/GCA Protein, Human (His)
Based on 2 publication(s) in Google Scholar
Grancalcin/GCA protein is a calcium-binding protein that plays a potential role in neutrophil adhesion to fibronectin and focal adhesion formation.As a homodimer, this protein exhibits interactions with SRI and LCP1, suggesting its involvement in molecular pathways related to cell adhesion and cytoskeletal dynamics.Grancalcin/GCA Protein, Human (His) is the recombinant human-derived Grancalcin/GCA protein, expressed by E.coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Grancalcin/GCA protein is a calcium-binding protein that plays a potential role in neutrophil adhesion to fibronectin and focal adhesion formation.As a homodimer, this protein exhibits interactions with SRI and LCP1, suggesting its involvement in molecular pathways related to cell adhesion and cytoskeletal dynamics.Grancalcin/GCA Protein, Human (His) is the recombinant human-derived Grancalcin/GCA protein, expressed by E.coli , with N-6*His labeled tag.
Background
Grancalcin (GCA) is a calcium-binding protein with putative functions in neutrophil adhesion to fibronectin and the formation of focal adhesions. Its role in calcium binding suggests involvement in signaling pathways that regulate cellular adhesion processes. GCA is known to form homodimers and has interactions with SRI and LCP1, further implicating its participation in protein-protein interactions that could modulate cellular functions. It has to emphasize GCA's potential involvement in mediating neutrophil adhesion to fibronectin and suggests a role in the establishment of focal adhesions, shedding light on its significance in cellular adhesion and signaling pathways.
Publications (2)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P28676 (M1-I217)
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Molecular Construction
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N-term
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6*His
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GCA (M1-I217)
Accession # P28676 -
C-term
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Protein Length
Full Length
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Synonyms
GCA; Grancalcin, EF-Hand Calcium-Binding Protein; Grancalcin; Grancalcin, EF-Hand Calcium Binding Protein; GCL; Grancalcin, Penta-EF-Hand Protein
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AA Sequence
MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI
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Predicted Molecular Mass
24 kDa
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Molecular Weight
Approximately 25-29 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 100 mM arginine, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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