Grancalcin/GCA Protein, Human (His)

Grancalcin/GCA protein is a calcium-binding protein that plays a potential role in neutrophil adhesion to fibronectin and focal adhesion formation. As a homodimer, this protein exhibits interactions with SRI and LCP1, suggesting its involvement in molecular pathways related to cell adhesion and cytoskeletal dynamics. Grancalcin/GCA Protein, Human (His) is the recombinant human-derived Grancalcin/GCA protein, expressed by E. coli , with N-6*His labeled tag. The total length of Grancalcin/GCA Protein, Human (His) is 217 a.a., with molecular weight of ~25 kDa.

Grancalcin (GCA) is a calcium-binding protein with putative functions in neutrophil adhesion to fibronectin and the formation of focal adhesions. Its role in calcium binding suggests involvement in signaling pathways that regulate cellular adhesion processes. GCA is known to form homodimers and has interactions with SRI and LCP1, further implicating its participation in protein-protein interactions that could modulate cellular functions. It has to emphasize GCA's potential involvement in mediating neutrophil adhesion to fibronectin and suggests a role in the establishment of focal adhesions, shedding light on its significance in cellular adhesion and signaling pathways.

Data is not available.

Human

E. coli

N-6*His

P28676 (M1-I217)

25801  [NCBI]

MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI

Approximately 25 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 200 mM NaCl, 100 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.