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Grancalcin/GCA Protein, Human (GST)

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Grancalcin/GCA protein is a calcium-binding protein that plays a potential role in neutrophil adhesion to fibronectin and focal adhesion formation.As a homodimer, this protein exhibits interactions with SRI and LCP1, suggesting its involvement in molecular pathways related to cell adhesion and cytoskeletal dynamics.Grancalcin/GCA Protein, Human (GST) is the recombinant human-derived Grancalcin/GCA protein, expressed by E.coli , with N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Grancalcin/GCA protein is a calcium-binding protein that plays a potential role in neutrophil adhesion to fibronectin and focal adhesion formation.As a homodimer, this protein exhibits interactions with SRI and LCP1, suggesting its involvement in molecular pathways related to cell adhesion and cytoskeletal dynamics.Grancalcin/GCA Protein, Human (GST) is the recombinant human-derived Grancalcin/GCA protein, expressed by E.coli , with N-GST labeled tag.

Background

Grancalcin (GCA) is a calcium-binding protein with putative functions in neutrophil adhesion to fibronectin and the formation of focal adhesions. Its role in calcium binding suggests involvement in signaling pathways that regulate cellular adhesion processes. GCA is known to form homodimers and has interactions with SRI and LCP1, further implicating its participation in protein-protein interactions that could modulate cellular functions. It has to emphasize GCA's potential involvement in mediating neutrophil adhesion to fibronectin and suggests a role in the establishment of focal adhesions, shedding light on its significance in cellular adhesion and signaling pathways.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-GST
  • Accession

    P28676 (M1-I217)

  • Gene ID
    25801  [NCBI]
  • Molecular Construction
    • N-term
    • GST
    • GCA (M1-I217)
      Accession # P28676
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    GCA; Grancalcin, EF-Hand Calcium-Binding Protein; Grancalcin; Grancalcin, EF-Hand Calcium Binding Protein; GCL; Grancalcin, Penta-EF-Hand Protein

  • AA Sequence

    MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI

  • Predicted Molecular Mass

    50.3 kDa

  • Molecular Weight

    Approximately 50 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 4% Sucrose, 4% mannitol, 0.02% Tween 80 (w/v), pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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